Can we use absolute methanol in the soxlet apparatus. And what is the best temperature for its use?
80% of methanol is best to use in soxhlet apparatus and best temperature is between 60 to 80°C
Yes you can use absolute methanol also. But 90% methanol is good for most of the purposes. Normally for efficient extraction, at least 72 cycles are required as a normal rule.You can check for the process completion be taking the cycling solvent (1-2 ml) on a watchglass and allowing it to evaporate. If you see some deposits on the glass, it means still the extraction is required and vice versa in case glass is clear.
Regards
I also use 70% methanol in water when doing Soxhlet extractions. But it still depends on the nature of your samples.
The quantity of the methanol is to be taken depends upon the amount of sample. Secondly the different percentages of methanol will extract the things in different proportion. It is also depends upon that which sample you are extracting. Sometimes, you have to take the sample and solvents based on the size of the Soxhlet apparatus you are using.
Its depend on just for your material and the active substances which you want
Choice of solvents / extractants for soxhlet extraction of plant materiall depends on the the nature of plant and chemical compounds it contain. However for gross extraction 80% methanol can be used.
80% of methanol is best to use in soxhlet apparatus and best temperature is between 60 to 80°C
Kindly please , how to remove the green color from the leaves extracts ??
@ Shumoos Ghanim
Pretreat the leaves with activated charcoal to remove the green color of chlorophyll.
@ Alok Nahata I tried using active charcol but it didnt remove it.
After extraction I add the activated charcol powder , then I filtrate the extract by filter paper. But the extract is still green.
Is there a different procedure ?
Thanks
Very first thing should be to remove the chlorophyll. So you should treat the leaves with activated charcoal instead of the extract. That would be more fruitful and easy too.
You have to boil the leaves with the charcoal for sometime. I hope you have done that. Cold treatment would not help too much.
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