How to find out the integrity of DNA after performing Real Time PCR?

Is it possible to find out the integrity of DNA using real time PCR for comparison among two different species?

02 March 2018 9,554 4 View

How to prepare the working stock if the primer concentration in 242.65pm/ul on addition of 200ul water?

03 April 2017 9,122 4 View

Is their any software available to design primer for inverse PCR?

I need to perform inverse PCR, is their any software available to design primers for inverse PCR? Thank You

31 December 2015 7,444 1 View

What are the important steps to perform overexpression of plant gene in pCambia 1301?

If we need to overexpress particular gene in pCambia 1301, do we need to do any changes in gene first before performing cloning. Thank You Raksha

07 August 2014 312 9 View

Is their any alternative to benzidine chemicals for the staining of peroxidase native PAGE?

To perform a native PAGE assay for peroxidase, what can be the chemical used for its staining?

04 May 2014 4,607 5 View

What is the best percentage of glycerol stock preparation for transformed culture recommended for the long term storage?

What is the best percentage of glycerol stock preparation for transformed culture is recommended for the long term storage? How long will cultures be efficiency maintained?

03 April 2014 3,349 26 View

To what extent is it advisable to use commercially available competent cells for transformation?

How much efficiency will remain in commercially available competent cells after one month?

11 December 2013 2,046 13 View

For Coding sequence trimming which online tool should be used?

To get good phylogenetic tree I need to get some sequences trimmed. I have searched for it but was unable to perform.

11 December 2013 6,373 2 View

Can anyone suggest me how to find out the CDS region from the FASTA sequence?

I got the sequence from the database in the FASTA format. I need to find the CDS region from the same. The sequence is from KEGG pathway database.

10 November 2013 7,317 10 View

How probable is the possibility of losing a vector culture if we grow it in only one antibiotic?

If a vector map is given two antibiotics and we will be growing it in only one antibiotic what are the possibilities to lose a plasmid?

09 October 2013 9,422 1 View

No title

02 March 2021 3,060 3 View

What do you know about biotechnology art?

What do you know about biotechnology art?

02 March 2021 1,653 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

What HPLC Marker(s) should be used to detect anthocyanins in a sample?

What HPLC Marker(s) or standard is used to detect and quantify anthocyanins such as Delphinidin, Cyanidin in a given sample of plant extract?

23 February 2021 1,832 4 View