15 Questions 5 Answers 0 Followers
Questions related from Oğuzhan Serçe
When designing an experimental setup and we want to buy a certain chemical, for example; let's say we want to buy penicillin; cas number: 61-33-6. Then we realize that we don't have enough money...
07 June 2021 3,460 3 View
Hello, I'm trying to make sgRNA plasmid vector for dCas9-VPR- AAV transducted system. In this vector that i shared in below, after screening tag, MCS is presented. I want to use these sites for...
08 October 2020 9,585 0 View
Hello, After we co-transfected our hek293 packaging cells with RC, pHelper, GOI for AAV production; can we stock some of them for future experiments with using freezing and thawing method? Are...
07 October 2020 3,924 1 View
Hello, I designed some systems related with double vector (for dCas9 VPR system and sgRNA casette). I wonder which one is better while applying them and is there any difference between them in...
07 October 2020 1,522 0 View
Hello, I am trying to do new aav2 strategy for dcas9 system. For vector backbones, i use addgene platform. But for aav serotypes, no information were added into addgene vector database, just Aav...
04 October 2020 9,612 3 View
Im looking for AAV type for p19 line (for best transduction eff.)
04 October 2020 726 0 View
I am not sure; is AAV2 best for SY5Y and HT22? Is there any comparative study for these lines? Also, Can i use same serotype vector for the two cell lines?
03 October 2020 3,551 2 View
I want to use ORI in my plasmid vector for replicating it in SH-SY5Y cells like in 293E or 293T cells with SV40? Is there any ORI that can be replicated in SY5Y cells?
25 September 2020 2,735 3 View
Can be episomal plasmid vector amplified in mitotic cells without host- suitable ORI such as SV40 in mammalian cell that express sv40 large T antigen?
25 September 2020 1,764 4 View
Many sources refers to epimutations as hereditary; but what if mutation just affect somatic cells (progenitor cells etc.) It shouldnt be heritable in this way. am I ignoring something?
30 January 2019 314 1 View
How do I find references to the size of the cDNA sequence I isolated?
13 March 2018 2,306 3 View
Late gast. fate is determined but organizer send factors for not committed cells. Environmental factors that organizers' induce might affect the committed cell duties. How we know that nothing...
09 March 2018 2,750 6 View
Can be possible the transcription factor's dna binding domain's binding to two or more different DNA sequence in different(or also same) enhancers? Or there is no exception, it must be specific...
24 February 2018 8,420 3 View
If we want cell to accept vector dna in transformation, we treated with calcium chloride or chilled on ice etc.. But i have a trouble with one issue about that. Books say that ; We must choose the...
15 December 2017 8,171 2 View
Is there an article about the fact that the sexual reproduction has existed; before asexual reproduction? I heard something new about it, but I can not find the article.
05 December 2017 419 5 View
