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When designing an experimental setup and we want to buy a certain chemical, for example; let's say we want to buy penicillin; cas number: 61-33-6. Then we realize that we don't have enough money...
07 June 2021 3,342 3 View
Hello, After we co-transfected our hek293 packaging cells with RC, pHelper, GOI for AAV production; can we stock some of them for future experiments with using freezing and thawing method? Are...
07 October 2020 3,871 1 View
Hello, I am trying to do new aav2 strategy for dcas9 system. For vector backbones, i use addgene platform. But for aav serotypes, no information were added into addgene vector database, just Aav...
04 October 2020 9,549 3 View
I am not sure; is AAV2 best for SY5Y and HT22? Is there any comparative study for these lines? Also, Can i use same serotype vector for the two cell lines?
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I want to use ORI in my plasmid vector for replicating it in SH-SY5Y cells like in 293E or 293T cells with SV40? Is there any ORI that can be replicated in SY5Y cells?
25 September 2020 2,647 3 View
Can be episomal plasmid vector amplified in mitotic cells without host- suitable ORI such as SV40 in mammalian cell that express sv40 large T antigen?
25 September 2020 1,665 4 View
How do I find references to the size of the cDNA sequence I isolated?
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Can be possible the transcription factor's dna binding domain's binding to two or more different DNA sequence in different(or also same) enhancers? Or there is no exception, it must be specific...
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