Hello,
I designed some systems related with double vector (for dCas9 VPR system and sgRNA casette).
I wonder which one is better while applying them and is there any difference between them in terms of convenience or efficiency?
I. AAV-dCas9VPR transduction (based on ITR-dCas9VPR-screening tag-ITR transfer plasmid) and then i am gonna screen them. After that, use plasmid vector which include sgRNA- screening tag (no viral based) and transfect it to transducted cells.
II. Another scenario that, ITR-dCaspVPR-GFP-ITR and ITR-sgRNA-RFP-ITR vectors are prepared for seperate viral particles in different hek293 producer plates,
a) I m gonna use them for same time transduction
or
b)I m gonna use them in order as one by one ( but i am worried about transient effect of AAV)
I may have asked very simple questions because I have no practical knowledge. I apologize to everyone in advance.
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