Can be episomal plasmid vector amplified in mitotic cells?

More Oğuzhan Serçe's questions See All

How can I find a dataset of pathological EMG signals ?

The importance of disease classification studies for the development of diagnosis & prognosis systems is crucial. Here the main problem is the lack of available medical datasets to use. For...

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Cell lines related with receptors, enzymes etc?

How i search a xxxx cell line related with enzymes, receptors, disease and such as well pathway? i have only cell line code and name.

07 April 2022 5,624 2 View

Why AF488 anti-human FoxP3 antibodies are used in Treg staining kits instead of FITC conjugated?

Dear all, I realized that Biolegend has all its Treg staining kits as AF488 conjugated FoxP3 and other dyes for CD25/CD4. What is the reason behind it that they are changing CD25 and CD4...

12 August 2021 3,568 2 View

How important are Cas numbers to the experimental design?

When designing an experimental setup and we want to buy a certain chemical, for example; let's say we want to buy penicillin; cas number: 61-33-6. Then we realize that we don't have enough money...

06 June 2021 3,440 3 View

Is this vector can be used as sgRNA vector?

Hello, I'm trying to make sgRNA plasmid vector for dCas9-VPR- AAV transducted system. In this vector that i shared in below, after screening tag, MCS is presented. I want to use these sites for...

07 October 2020 9,563 0 View

After we co-transfected our hek293 packaging cells for AAV can we stock some of them for future experiments with using freezing & thawing?

Hello, After we co-transfected our hek293 packaging cells with RC, pHelper, GOI for AAV production; can we stock some of them for future experiments with using freezing and thawing method? Are...

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Which one is the better strategy for AAV transfer plasmids?

Hello, I designed some systems related with double vector (for dCas9 VPR system and sgRNA casette). I wonder which one is better while applying them and is there any difference between them in...

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Is there any AAV serotype that can be transduced to p19 cell line?

Im looking for AAV type for p19 line (for best transduction eff.)

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Do any aav vectors include serotype info?

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Which AAV serotype is the best for SY5Y and HT22 cells?

I am not sure; is AAV2 best for SY5Y and HT22? Is there any comparative study for these lines? Also, Can i use same serotype vector for the two cell lines?

02 October 2020 3,498 2 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

How can I apply boundary conditions in an orthotropic steel deck numerical model using ABAQUS software?

I am trying to simulate vehicular loading on an orthotopic steel deck bridge section in ABAQUS software. The red arrow mark in the attached figure indicates the direction in which the vehicle will...

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Unusual intensity drop in some sections of chromatograms in DDA?

Hi, we have measured tryptic peptides using both DDA and DIA method on QExactive. In DDA replicates i saw unusual intensity drops occurring at the same sections of chromatograms in DDA replicates...

07 August 2024 3,218 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Where should the ARS sequence be introduced on a plasmid?

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05 August 2024 1,573 4 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

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Why my colony PCR results of my recombinant bacterial not showing any results?

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05 August 2024 2,570 3 View

How can we now if the promotor in the vector is well?

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Transfection in HEK293T cells?

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31 July 2024 9,892 4 View

Issues with Protein Expression in Transfected cells?

Hello I am trying to create a stable cell line in HEK293 via Lipofectamine 3000 transfection. My plasmid is a CD63-IL10-GFP construct with Puromycin resistance. I am successful with the...

30 July 2024 6,648 1 View

Michael Halim Popular answer

Interesting question. Following the discussion.

Walid Hassene Hamri

Hi,

You may find what you looking for in the link below:

Article Episomal vectors for gene expression in mammalian cells

John Schloendorn

This can often work. For example, CHO cell lines for transient expression are made in this way.

Michael Halim