Hello,
I am trying to do new aav2 strategy for dcas9 system. For vector backbones, i use addgene platform. But for aav serotypes, no information were added into addgene vector database, just Aav was written in data for GOI backbones.
For GOI vector, serotype specificity is not necessary ?
Hello,
It's probably because the AAV serotype is not dependent of the GOI backbone. This backbone only contain ITR which are from AAV2. ITR from AAV2 are used to produced all serotypes of rAAV vector (except AAV3 I think). The capsid proteins are encoded in an other plasmid. Hope it's help you.
Hi, Indeed, as Rebecca pointed out, the serotype is determined by the capsid and not by the AAV genome. AAV vector plasmids (in which GOI is inserted) are usually from AAV2, but the capsid proteins that are encoded by your "packaging" plasmids can be from another serotype. If you want to find out the serotype, check info on your helper plasmids.
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