deleted*deleted*deleted*deleted deleted deleted?

More Oğuzhan Serçe's questions See All

How can I find a dataset of pathological EMG signals ?

The importance of disease classification studies for the development of diagnosis & prognosis systems is crucial. Here the main problem is the lack of available medical datasets to use. For...

31 October 2023 1,151 4 View

Cell lines related with receptors, enzymes etc?

How i search a xxxx cell line related with enzymes, receptors, disease and such as well pathway? i have only cell line code and name.

07 April 2022 5,624 2 View

Why AF488 anti-human FoxP3 antibodies are used in Treg staining kits instead of FITC conjugated?

Dear all, I realized that Biolegend has all its Treg staining kits as AF488 conjugated FoxP3 and other dyes for CD25/CD4. What is the reason behind it that they are changing CD25 and CD4...

12 August 2021 3,568 2 View

How important are Cas numbers to the experimental design?

When designing an experimental setup and we want to buy a certain chemical, for example; let's say we want to buy penicillin; cas number: 61-33-6. Then we realize that we don't have enough money...

06 June 2021 3,440 3 View

Is this vector can be used as sgRNA vector?

Hello, I'm trying to make sgRNA plasmid vector for dCas9-VPR- AAV transducted system. In this vector that i shared in below, after screening tag, MCS is presented. I want to use these sites for...

07 October 2020 9,563 0 View

After we co-transfected our hek293 packaging cells for AAV can we stock some of them for future experiments with using freezing & thawing?

Hello, After we co-transfected our hek293 packaging cells with RC, pHelper, GOI for AAV production; can we stock some of them for future experiments with using freezing and thawing method? Are...

06 October 2020 3,896 1 View

Which one is the better strategy for AAV transfer plasmids?

Hello, I designed some systems related with double vector (for dCas9 VPR system and sgRNA casette). I wonder which one is better while applying them and is there any difference between them in...

06 October 2020 1,501 0 View

Is there any AAV serotype that can be transduced to p19 cell line?

Im looking for AAV type for p19 line (for best transduction eff.)

03 October 2020 706 0 View

Do any aav vectors include serotype info?

Hello, I am trying to do new aav2 strategy for dcas9 system. For vector backbones, i use addgene platform. But for aav serotypes, no information were added into addgene vector database, just Aav...

03 October 2020 9,589 3 View

Which AAV serotype is the best for SY5Y and HT22 cells?

I am not sure; is AAV2 best for SY5Y and HT22? Is there any comparative study for these lines? Also, Can i use same serotype vector for the two cell lines?

02 October 2020 3,498 2 View

Basis set and input instruction to calculate HOMO and LUMO using Orca for Imidazolium based organic salts?

Hey there, As a synthetic chemist delving into theoretical calculations for my imidazolium-based organic molecules, I would greatly appreciate any guidance on the appropriate input instructions...

09 August 2024 5,444 7 View

Is the mentioned CV graph a valid one as this graph have only one peak prominent (reduction)?

I have used Prussian blue nanoparticles as a redox couple. The PBNPs have been made using only one salt precursor. Also, during scan rate studies, a small oxidation peak can be consistently found...

31 July 2024 9,697 0 View

How to remove/ replace trifluoroacetate countertop after deprotection?

After deprotection of the tert-butyl ester to generate the carboxylic acid in chloroform with TFA, my compound‘s pyridine moiety forms a TFacetate ammonium salt (small molecule drug, not a...

29 July 2024 9,753 3 View

How do nutrient transporters and aquaporins interact under abiotic stress in plants?

"The Correlation between Nutrition and Transport Mechanism under Abiotic Stress in Plants: A Comprehensive Review" by Muhammad Saleem, Jianhua Zhang, Muhammad Qasim, Rashid Iqbal, and Li Song,...

23 July 2024 9,109 0 View

How to prepare polyaniline emeraldine salt ( PANI -ES) sample for Transmission electron microscope (TEM) analysis?

I need to do TEM analysis for my Polyaniline emeraldine salt form. May I know which solvent is appropriate for preparing TEM sample?

25 June 2024 9,086 3 View

Challenges in Stabilizing and Concentrating a Highly Unstable Acetyltransferase for Crystallization?

I am working on an acetyltransferase that is highly unstable. Its pI is 6.65, and its molecular weight is around 18 kDa. The protein elutes at 1M imidazole and begins to precipitate immediately...

19 June 2024 2,590 0 View

Which media will produce more biomass in saccharomyces cerevisiae?

Dear all, For higher biomass for saccharomyces cerevisiae which media is suitable in shake flask? 1. YPD media 2. Basal salt media and what is the optimum C/N ratio ( 5:1 /10:1). Thanks &...

18 June 2024 2,106 0 View

How can I prepare 30m ZnCl2 aqueous solution?

A 30m ZnCl2 aqueous solution is needed to prepare and use the electrolyte, as used in some references denoted below. [Chen, Ze, et al. Journal of the American Chemical Society 145.37 (2023):...

11 June 2024 4,479 1 View

Hello dear researchers Is there an alternative for gold chloride salt in electrochemical biosensor studies?

The price of gold chloride salt is unfortunately very high.

08 June 2024 1,469 0 View

How to prepare 1000 ppm NO3 (nitrate) solution using KNO3 salt ?

I need the calculation procedure for this Thank You

05 June 2024 2,752 2 View

Riaz A. Khan

The CAS number is the actual ID of that very compound. If you feel you can use a replacement, use it but you will need to declare the actual compound/starting material/reagent/synthon you used with its CAS number. No two compounds CAS number can be exchanged.

Steingrimur Stefansson

Nominated for the most inane question of the week.

Christian G Meyer

Arrogant posts are never helpful.