Can i use ORI in my plasmid vector for replicating it in SH-SY5Y cells?

I want to use ORI in my plasmid vector for replicating it in SH-SY5Y cells like in 293E or 293T cells with SV40? Is there any ORI that can be replicated in SY5Y cells?

24 September 2020 2,541 3 View

Can be episomal plasmid vector amplified in mitotic cells?

Can be episomal plasmid vector amplified in mitotic cells without host- suitable ORI such as SV40 in mammalian cell that express sv40 large T antigen?

24 September 2020 1,570 4 View

How do you dissolve and inject indomethacin for rat studies?

I dissolved indomethacin (Santa Cruz Biotech - SC-200503) in DMSO and prepared stock of 25 mg/mL In my study, I inject 10 mg/kg i.p., injection volume 0,5 mL and I work with 250-300 g rats. When...

13 September 2020 5,659 3 View

Why do i get wrong total pressure data from Ansys Fluent?

Hi everyone, I have been working on a laminar compressible microtube flow analysis (supercritical fluid). Mass flow inlet, pressure outlet and constant wall heat flux boundary conditions are...

17 December 2019 1,403 4 View

How can cyclic symmetry be defined in dynamic, implicit analyse in Abaqus?

Hi, I need some support to define cyclic symmetry in dynamic, implicit analyse. As I have found out that cyclic symmetry under interactions is only usable in static, visco, frequency, steady...

26 November 2018 3,020 5 View

How do I find references to the size of the cDNA sequence I isolated?

How do I find references to the size of the cDNA sequence I isolated?

12 March 2018 2,101 3 View

Can be possible the dna binding domain's binding to two or more different DNA sequence in different enhancers?

Can be possible the transcription factor's dna binding domain's binding to two or more different DNA sequence in different(or also same) enhancers? Or there is no exception, it must be specific...

23 February 2018 8,240 3 View

Why is ETOTAL negative in ABAQUS Quasi Static Analyse?

Hi, I did an quasi static analyse. As a result of my analysis, total energy of the output set (etotal) is everytime negative and after some steps etotal increases exponentially in negative area....

23 January 2018 7,094 6 View

How can I find out the rotational stiffness of the bearing with static structural analysis?

Hi, I have some problem about the rotational stiffness of the bushing. Actually, I would like to change the bearing balls with bushing joints in ANSYS Workbench. I have a normal bearing with the...

01 November 2016 7,088 3 View

How can I remove orientation node from beam element in ANSYS WB?

Hi, I have a problem with orientation nodes of beam element in ANSYS. My Structure: - 3D Structure (Every node have 6 DOF) - 12 Beam Elements - 13 Nodes - 2 Fixed supports at the edges - After...

24 February 2016 541 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

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02 March 2021 3,060 3 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View