Which AAV serotype is the best for SY5Y and HT22 cells?

More Oğuzhan Serçe's questions See All

How can I find a dataset of pathological EMG signals ?

The importance of disease classification studies for the development of diagnosis & prognosis systems is crucial. Here the main problem is the lack of available medical datasets to use. For...

31 October 2023 1,151 4 View

Cell lines related with receptors, enzymes etc?

How i search a xxxx cell line related with enzymes, receptors, disease and such as well pathway? i have only cell line code and name.

07 April 2022 5,624 2 View

Why AF488 anti-human FoxP3 antibodies are used in Treg staining kits instead of FITC conjugated?

Dear all, I realized that Biolegend has all its Treg staining kits as AF488 conjugated FoxP3 and other dyes for CD25/CD4. What is the reason behind it that they are changing CD25 and CD4...

12 August 2021 3,568 2 View

How important are Cas numbers to the experimental design?

When designing an experimental setup and we want to buy a certain chemical, for example; let's say we want to buy penicillin; cas number: 61-33-6. Then we realize that we don't have enough money...

06 June 2021 3,440 3 View

Is this vector can be used as sgRNA vector?

Hello, I'm trying to make sgRNA plasmid vector for dCas9-VPR- AAV transducted system. In this vector that i shared in below, after screening tag, MCS is presented. I want to use these sites for...

07 October 2020 9,563 0 View

After we co-transfected our hek293 packaging cells for AAV can we stock some of them for future experiments with using freezing & thawing?

Hello, After we co-transfected our hek293 packaging cells with RC, pHelper, GOI for AAV production; can we stock some of them for future experiments with using freezing and thawing method? Are...

06 October 2020 3,896 1 View

Which one is the better strategy for AAV transfer plasmids?

Hello, I designed some systems related with double vector (for dCas9 VPR system and sgRNA casette). I wonder which one is better while applying them and is there any difference between them in...

06 October 2020 1,501 0 View

Is there any AAV serotype that can be transduced to p19 cell line?

Im looking for AAV type for p19 line (for best transduction eff.)

03 October 2020 706 0 View

Do any aav vectors include serotype info?

Hello, I am trying to do new aav2 strategy for dcas9 system. For vector backbones, i use addgene platform. But for aav serotypes, no information were added into addgene vector database, just Aav...

03 October 2020 9,589 3 View

Can be episomal plasmid vector amplified in mitotic cells?

Can be episomal plasmid vector amplified in mitotic cells without host- suitable ORI such as SV40 in mammalian cell that express sv40 large T antigen?

24 September 2020 1,743 4 View

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,115 1 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Are there any instruments for studying time similar to the way it is in space?

There are a huge number of methods for studying objects in space, according to the senses (and not only). Mechanical, thermal, optical, acoustic, electrical, magnetic, based on particle beams,...

06 August 2024 7,102 0 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

How to report results of Generalised Linear Mixed Models in a journal article?

Hi everyone, If you have written or come across any papers where Generalised Linear Mixed Models are used to examine intervention (e.g., in mental health) efficacy, could you please share the...

04 August 2024 4,130 4 View

Guochao Wu

Hi Oğuzhan,

Please see the paper, this may be helpful.

Article A survey of ex vivo/in vitro transduction efficiency of mamm...

Andrew Osborne

AAV2 would be a sensible first option. I've used AAV2 to transduce SY5Y cells before. AAV-DJ would be my other recommendation and often results in higher transduction of a broader range of cell lines.