Can i use ORI in my plasmid vector for replicating it in SH-SY5Y cells?

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Michael Halim Popular answer

Interesting question. Following the discussion.

Yoav Lubelsky

Mammalian replication origins are poorly defined, and usually consist of large regions. There is no equivalent to yeast ARS sequences or bacterial Oris that can be inserted into a plasmid and allow it to be replicated.

The SV40 ori/T-antigen is the exception.

Michael Halim