If we want cell to accept vector dna in transformation, we treated with calcium chloride or chilled on ice etc.. But i have a trouble with one issue about that. Books say that ; We must choose the vector that specific according to the cell that will do transformation. But we will treat with calcium chloride and ice, why we choose specific vector?, this foreign vector can be enforced the cell for accepting. I know cell might accept the vector as a homolog sequence and might do cross with this vector, might take homolog genes into own dna, but it doesnt have to be. Vector might stay in the cytoplasm and i think it dont require the homolog sequences with cell's own DNA . Transformation should occur spontaneously due to bacteria nature, already
Not all plasmids can be introduced in all hosts. Sometimes the host strain already carries the selection marker borne on the plasmid. This happens, for instance, in Novagen's Origami strain, which is KmR if memory serves well. Sometimes you are trying to propagate a positive selection vector, in which case you have to use a host where the positive selection scheme is not operational. Or the host strain might be harboring a plasmid with an incompatible replicon.
Hope it helps!
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