Im looking for AAV type for p19 line (for best transduction eff.)
The importance of disease classification studies for the development of diagnosis & prognosis systems is crucial. Here the main problem is the lack of available medical datasets to use. For...
31 October 2023 1,151 4 View
How i search a xxxx cell line related with enzymes, receptors, disease and such as well pathway? i have only cell line code and name.
07 April 2022 5,624 2 View
Dear all, I realized that Biolegend has all its Treg staining kits as AF488 conjugated FoxP3 and other dyes for CD25/CD4. What is the reason behind it that they are changing CD25 and CD4...
12 August 2021 3,568 2 View
When designing an experimental setup and we want to buy a certain chemical, for example; let's say we want to buy penicillin; cas number: 61-33-6. Then we realize that we don't have enough money...
06 June 2021 3,440 3 View
Hello, I'm trying to make sgRNA plasmid vector for dCas9-VPR- AAV transducted system. In this vector that i shared in below, after screening tag, MCS is presented. I want to use these sites for...
07 October 2020 9,563 0 View
Hello, After we co-transfected our hek293 packaging cells with RC, pHelper, GOI for AAV production; can we stock some of them for future experiments with using freezing and thawing method? Are...
06 October 2020 3,896 1 View
Hello, I designed some systems related with double vector (for dCas9 VPR system and sgRNA casette). I wonder which one is better while applying them and is there any difference between them in...
06 October 2020 1,501 0 View
Hello, I am trying to do new aav2 strategy for dcas9 system. For vector backbones, i use addgene platform. But for aav serotypes, no information were added into addgene vector database, just Aav...
03 October 2020 9,589 3 View
I am not sure; is AAV2 best for SY5Y and HT22? Is there any comparative study for these lines? Also, Can i use same serotype vector for the two cell lines?
02 October 2020 3,498 2 View
Can be episomal plasmid vector amplified in mitotic cells without host- suitable ORI such as SV40 in mammalian cell that express sv40 large T antigen?
24 September 2020 1,743 4 View
I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?
11 August 2024 3,115 1 View
Hello everyone, I am researching ways to enhance the quality of lamb milk replacer for machine feeding. I would appreciate any insights or recommendations on the following: Manual vs. Machine...
06 August 2024 6,227 2 View
I need the python code to forecast what crop production will be in the next decade considering climate and crop production variables as seen in the attached.csv file.
05 August 2024 2,977 3 View
What are the biological importance of bacteria and the role of microbes to improve crop productivity and soil health and role of bacteria as decomposers in cycling nutrients?
31 July 2024 3,479 5 View
What is the role of microorganisms in soil fertility and crop production cycles and role of microorganisms in mineral transformation?
31 July 2024 8,483 8 View
poultry production in nigeria
29 July 2024 5,518 2 View
I am looking to predict some production for future onshore wind energy production in Portugal, is there any database with info of performances of the existing wind farms in the country? something...
28 July 2024 1,750 1 View
Contemporary scores or innovations in scientific approaches. Hybrid methodologies, emerging themes and cross-cutting issues?
27 July 2024 648 3 View
List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?
27 July 2024 4,800 4 View
Hi, I need to prepare a standard curve of H2O2 to evaluate the primary products of oxidation. H2O2 I have is 35%. How do I prepare a stock of H2O2 10 microlitere. If s.o has experience, I would be...
26 July 2024 3,460 2 View
