Im looking for AAV type for p19 line (for best transduction eff.)
I am not sure; is AAV2 best for SY5Y and HT22? Is there any comparative study for these lines? Also, Can i use same serotype vector for the two cell lines?
02 October 2020 3,328 2 View
I want to use ORI in my plasmid vector for replicating it in SH-SY5Y cells like in 293E or 293T cells with SV40? Is there any ORI that can be replicated in SY5Y cells?
24 September 2020 2,541 3 View
Can be episomal plasmid vector amplified in mitotic cells without host- suitable ORI such as SV40 in mammalian cell that express sv40 large T antigen?
24 September 2020 1,570 4 View
I dissolved indomethacin (Santa Cruz Biotech - SC-200503) in DMSO and prepared stock of 25 mg/mL In my study, I inject 10 mg/kg i.p., injection volume 0,5 mL and I work with 250-300 g rats. When...
13 September 2020 5,659 3 View
Hi everyone, I have been working on a laminar compressible microtube flow analysis (supercritical fluid). Mass flow inlet, pressure outlet and constant wall heat flux boundary conditions are...
17 December 2019 1,403 4 View
Hi, I need some support to define cyclic symmetry in dynamic, implicit analyse. As I have found out that cyclic symmetry under interactions is only usable in static, visco, frequency, steady...
26 November 2018 3,020 5 View
How do I find references to the size of the cDNA sequence I isolated?
12 March 2018 2,101 3 View
Can be possible the transcription factor's dna binding domain's binding to two or more different DNA sequence in different(or also same) enhancers? Or there is no exception, it must be specific...
23 February 2018 8,240 3 View
Hi, I did an quasi static analyse. As a result of my analysis, total energy of the output set (etotal) is everytime negative and after some steps etotal increases exponentially in negative area....
23 January 2018 7,094 6 View
Hi, I have some problem about the rotational stiffness of the bushing. Actually, I would like to change the bearing balls with bushing joints in ANSYS Workbench. I have a normal bearing with the...
01 November 2016 7,088 3 View
I am a Petroleum Production Engg MSc student at Robert Gordon University in Aberdeen and I am in the process of selecting a topic for my MSc Engineering Investigation proposal. I would very much...
01 March 2021 7,396 4 View
Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...
28 February 2021 2,723 4 View
I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...
28 February 2021 4,949 3 View
The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...
28 February 2021 6,560 14 View
When using a lentiviral vector for inducible expression of genes using the Tet-On system. In the literature, I saw a widely used of pLVX-TetOne-Puro Plasmid, is there a reason why people prefer to...
25 February 2021 1,011 3 View
Hi everyone How to model Supercritical water gasification (SCWG) process in Homer software?
25 February 2021 4,075 1 View
I have been trying to have a good AAV titration for my production and I have tried different plasmids to set up as a standard curve but each one behaves differently. i am trying to use ITR for...
23 February 2021 5,167 3 View
Secretory universal influenza antibody CR9114 were successfully expressed by adenovirus vector ,then we used protein A G beads purify CR9114.But the molecular weight of the purified antibody under...
22 February 2021 4,318 2 View
My real system (buck converter) can only take an input of 0 to 1 (duty ratio) and I need to constrain the system so the controller action keeps within this bounds. How can I do this? The code for...
22 February 2021 2,125 3 View
Would you please let me to know about the methods for measurement of medullation and Kemp % on animal? My problem is that the wool sampling and sending to laboratory is not possible. So I would...
21 February 2021 7,260 4 View
