14 Questions 24 Answers 0 Followers
Questions related from Muhammad Arslan
Just wondering if someone has done it? We usually use environmental DNA (eDNA) for library preparation and I am wondering if eDNA concentration is very low, can we initially amplify the 16S region...
19 April 2021 2,807 1 View
Hi all, As we all know that 16S amplicon seq data by Illumina sequencing is semi-quantitative and therefore I want to normalize my data output with the qPCR 16S data. Can you please recommend me...
27 July 2018 1,925 3 View
Hi all, I am trying to calculate organic loading rate for the vertical flow constructed wetland. I have COD of 493 mg/L with the flow rate 0.5 m3/d. Multiplication gives me 246.5 kg COD/m3/d. But...
27 June 2018 5,770 3 View
Dear all,I am writing this message to ask if you can comment on layering multiple images in 3d-space (cf attachment). I have confocal images and wants to do better presentation. Your comment would...
06 June 2017 4,436 3 View
Hi all, I want to do rarefaction analysis for the plant "Juncus effusus" transcriptome assembly. At the moment I have Fasta file with final assembly. I wonder how can I use this dataset? Is there...
30 May 2017 1,457 2 View
Hi all, Can you suggest me a method to create a heat map based on actual data and not the relative percentages? I need it because I have different treatments with increasing the overall...
17 February 2017 1,034 3 View
Hi all, I need expert opinions on this matter as my plant samples are detached during washing step of FISH. I used agarose as well as transparent nail polish for this purpose and to me agarose...
11 August 2016 6,005 19 View
Hi All, I want to ask if we are trying to amplify plant endophytes (specifically firmicutes) from the bulk DNA (extracted by MPbio kit), how can we check the binding of primers with chloroplast...
01 July 2016 805 3 View
If we want to determine the copy number (by qPCR) of all microorganisms within plant tissues, how can we avoid the chloroplast and mitochondrial DNA! Thanks
13 May 2016 2,728 2 View
Briefly, I am looking to test if there is some pathogenicity caused by (endophytic) bacteria residing within plant tissues. What methods one can adopt to test it in situ? Moreover, please comment...
12 April 2016 9,387 1 View
Hi All, I want to perform meta-analysis for comparison between control and experiment group but most of the times i find only percentages data and not actual data-set! Do you think that it would...
19 March 2016 2,669 6 View
We have sequenced a strain of Anoxybacillys flavithermus AK1. Now, i want to prepare circular map of whole genome using DNA Plotter. The software can load only one fasta file which is why I need...
15 May 2015 3,335 14 View
We have sequenced a strain of Anoxybacillys flavithermus AK1. Now, i want to prepare circular map for our paper. While searching, i found software DNA plotter which is really good. However, it has...
14 May 2015 8,189 21 View
Actually, I want to find the the16S rRNA for "Anoxybacillus flavithermus strain TNO-09.006". Its whole genome sequence is present in the database but i want 16S rRNA sequence to do phylogeny along...
11 March 2015 3,607 9 View
