Any good way to normalize 16S amplicon illumina-seq data with qPCR data?

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Abhijeet Singh

@ Ajit kumar Roy - can you please explain how the link suggested by you solves the question ?

@ Muhammad Arslan - have you considered doing RT-qPCR using the standard curves of total bacterial/archeael plasmids at several concentrations ? I dont think illumina data and qPCR could be normalized in any other way, but would like to see other answers.

Muhammad Arslan

Thanks Abhijeet. Indeed this is a good argument. But I am not sure what do you mean by several concentrations. Are you referring to inhibition factor? If yes, then I do have 3 concentrations of qPCR for each reaction. 1. actual, 2. 1/100, and 3. 1/1000

Actual concentration didnt work so good but the other two did work.

Thanks for your comment.

Yes.... you got it right. With the possibility of inhibition, several concentrations would be a good strategy.