Just wondering if someone has done it? We usually use environmental DNA (eDNA) for library preparation and I am wondering if eDNA concentration is very low, can we initially amplify the 16S region (1600 bp) via 27F and 1492R primers and then use the PCR prodcut for library preparation. Would be glad to hear from your experiences.

Thanks

PS: the concentration of eDNA is low and doesn't match the quality control criteria for a private sequencing company.