i am very skeptical about the idea that a pipettes life increases if it is put on max after use. is here any actual data on this?
Hi i have tried through literature search find out how "bendy" m13 phage is. and so fare i have come back with nothing. there are papers describing how much the phage can stretch but nothing...
17 January 2024 2,270 0 View
if i wanted to collect a clinical sample from healthy individuals of different age groups including older adults, do i need different exclusion criteria for healthy between age groups? What...
16 January 2024 5,742 0 View
Most phage display selections are made in 2-5 rounds, meaning that the phages that bind to the target gets amplified through growth in bacteria between each round. But PhIP-seq, which is phage...
19 December 2023 8,532 0 View
Hi i am starting working with insect cell protein expression from previously working with bacterial protein expression. for bacteria you can pellet and then freeze the cells after expression...
10 April 2023 8,295 2 View
hi it seems that my lab has some kind of lytic ohage contamination from some where. does anyone have any good cleaning ideas to get rid of them? we autoclaved the pipetts and cleaned all surfaces...
10 July 2022 6,847 2 View
Is there a way to regenerate streptavidin magnetic beads? I am guessing no because i am guessing when i elute i break the interaction between my biotinylated peptide and the protein not between...
16 November 2021 5,448 3 View
I have neutravidin and want to immobilize it on 96 well maxisorp plates so i can bind biotinulated peptides to it for elisa. does anyone have a good protocol for this? what buffer, good...
07 October 2021 7,447 1 View
So we have an FPLC system which is the P900 that has been acting wierd for a week or two. Even though no air should be in the system the pump introduces air anyways and makes the clicking noise...
16 March 2021 3,451 2 View
Hi I have a protein labeled with rhodamine B and after reaction i used Zeba spin column for dye removal. my problem is that lot of unreacted dye remained which interfered with my florescent...
02 February 2021 897 2 View
Hi I want to label a protein with Rhodamine B Isothiocyanate which failed. I have the protein in PBS pH 7.2 the Rhodamine B Isothiocyanate dissolved in DMSO at 10 mM. added 15 times molar excess...
26 January 2021 7,752 3 View
After World War, we have seen the formation of the United Nations. The sovereign body for peacekeeping but with regards to the Russia-Ukraine war it is visible that the UN seems to be ineffective...
18 July 2024 4,674 4 View
I've been fabricating traction force microscopy devices in glass bottom dishes, using a method that first spins a 100 micron PDMS layer, then a ~1 micron PDMS + fluorescent beads mixture layer....
09 June 2024 652 2 View
While running sdevice mode in sentaurus TCAD, the status doesn't change from running, so I checked the out file and it seems to change from line 18000 to line1000 and doesn't go back after that....
29 May 2024 5,620 0 View
What organism is responsible for recycling nitrogen back into the environment and organism is responsible for recycling nitrogen back into the environment?
13 May 2024 2,646 3 View
https://www.researchgate.net/profile/Peter-Messer-2/research My article entitled "A Review of Nearctic Selenophorus ..." now can't be sent to people requesting via ResearchGate. Error message:...
10 May 2024 8,402 15 View
We are intended to write a review articles in the domain of Composites, Computational Composite Mechanics, Composites Applications in Aerospace, and Composites trends in Renewable...
10 May 2024 9,585 1 View
I want to remove my article on Quantum key distribution from preprint version on your we bsite.
08 May 2024 2,383 1 View
Hello. I am Bakhtier Rasulov. For many years I have been a member of the scientific and social network ResearchGate. My scientific publications amounting to about 50 articles were presented there....
05 May 2024 1,287 1 View
I wanted to ask if there is a difference between connected components analysis and pore network analysis? I am trying to find porosity of a coating on a coated sample and I want to know which...
25 April 2024 2,992 1 View
How do microorganisms that cause decay decomposition release carbon dioxide back to the atmosphere and microbes are used to help reduce carbon buildup in the atmosphere?
14 April 2024 9,043 6 View