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Questions related from Go J Yoshida
Given the recent case report by NEJM (Pallais JC, Fenves AZ, Lu MT, Glomski K. Case 18-2018: A 45-Year-Old Woman with Hypertension, Fatigue, and Altered Mental Status. N Engl J Med....
07 July 2018 1,739 1 View
I have recently wondered whether the enhanced SA-beta-gal staining alone really reflects the cellular senescence.
06 June 2018 1,619 0 View
Given that multiple myeloma is a monoclonal neoplasm which is originated from plasma cells, there is a controversy whether there exist cancer stem-like cells in MM or not. Could you give me your...
04 April 2018 4,347 0 View
I think that cancer cells undergo "evolutionary dynamics" after chemotherapy and radiation therapy as the selective pressure. Excessive amount of reactive oxygen species (ROS) corresponds to one...
01 January 2018 1,125 1 View
When you isolate endothelial cells of the blood vessels, using only CD31 antibody is not enough to isolate them? Why alpha-smooth muscle actin (alpha-SMA) antibody is necessary according to some...
01 January 2018 2,653 2 View
It has been reported that the animal experiments using rats have revealed the emergence of lung cancer, mainly bronchioalveolar carcinoma and combined carcinoma and adenoma after the exposure to...
01 January 2018 9,208 0 View
We have reported several years ago the 5-year-old male patient with Li-Fraumeni syndrome with osteosarcoma and atypical type of hepatic cancer. Intriguingly, the cancer stem cell marker CD44...
01 January 2018 4,948 2 View
I have reported that chemotherapy for the patient with Li-Fraumeni syndrome results in the ectopic CD44 variant-positive cancer stem cells. Given that chemotherapy often increases the ROS level,...
01 January 2017 6,232 3 View
Neurotransmitters have been shown to drive tumor progression in the prostate glands and the stomach. In particular, it has recently been shown that acetylcholine promotes proliferation of...
01 January 2017 1,127 5 View
The investigators derived, from a human metastatic lung-cancer cell line and a breast-cancer cell line, latency-competent cells that seed distant sites after experimental tail-vein inoculation...
07 July 2016 8,223 0 View
While Drp1 contributes to mitochondrial fission, OPA1 has been widely recognized as to promote mitochondrial fusion in cooperative manner with Mfn1/2. Several papers have shown the representative...
06 June 2016 7,705 2 View
With Anti-xCT antibody (ab37185), I have obtained ever time the two separate bands composed of 55kDa and 37kDa. Many papers insist that 37kDa is the correct one, but as far as si-RNA-mediated...
05 May 2016 897 3 View
TERT promoter mutations, IDH mutations and 1p/19q co-depletion are surely promising markers to classify clinically heterogeneous cases of glioma. Among Grade-IV gliomas patients, however, Figure1...
01 January 2016 4,054 1 View
I am confused with the two kinds of cohort analyses composed of discovery cohort and validation cohort shown in Figure2 e-h in the attached file.According to ROC curves, both analysis tells that...
07 July 2015 5,037 7 View
Sarcoidosis is widely known to be one of auto-immune diseases which cause several granulomas with giant multi-nuclear cells derived from macrophages. In the case of sarcoidosis, asteroid body...
05 May 2015 9,676 8 View
I would be appreciated if you are kind enough to tell me the detailed method of the fluorescent immunostaining of organoid model cultured in collagen-I or Matrigel. As far as my experience, it...
04 April 2015 1,238 16 View
I have recently found out that Gene X-knockout mice develop black footpads much the same way as shown in Figure1. Immunostaining with ITGA6 revealed that differentiated melanocytes proliferate in...
03 March 2015 8,772 1 View
I have difficulty in separating the phosporylated M-MITF band and total M-MITF isoform, both of which are approximately 60kDa. I have used the cocktail of C5 clone and D5 clone, both of which are...
02 February 2015 5,194 3 View
We have previously identified the decreased expression of HFSC markers such as Lhx2, ColXVII, CD34 and Sox2 in the aging mice. However, the number of HFSCs was not so different as compared with...
02 February 2015 5,005 12 View
I have recently investigated into the difference of MITF expression patterns among the human melanoma cancer cell lines composed of SK-MEL-28, MeWo, 501MEL, and A375. These cells predominantly...
02 February 2015 9,624 4 View
I have recently been interested in MITF and its interaction with tyrosine kinase receptor family and performed several experiments using melanoma cell lines and xenograft model. However, according...
02 February 2015 8,178 1 View
We are interested in the dynamics of quiescent and proliferative cancer stem cells (CSCs) when tumor tissue shows invasion. We have previously investigated into the expression level of CD44...
01 January 2015 1,585 4 View
Multiple myeloma (MM) is a clonal bone marrow disease characterized by the neoplastic transformation of differentiated B cells. There are several papers focusing on cancer stem cells in MM....
01 January 1970 439 1 View
It is well known that autoantibody against aquaporin 4 (AQP4) is responsible for NMOSD (neuromyelitis Optica spectrum disorder). However, there are several NMOSD cases in which the titer of...
01 January 1970 2,682 1 View
It is widely accepted that AQP1, 4, and 9 are expressed in the brain. Among of them, AQP9 belongs to the aquaglyceroporin which is involved in the lipid metabolism. Given that AQP3, 7, and 9...
01 January 1970 4,714 8 View
