Does the enhanced SA-beta-gal staining alone really reflect the cellular senescence?

I have recently wondered whether the enhanced SA-beta-gal staining alone really reflects the cellular senescence.

05 June 2018 1,495 0 View

There is a cancer stem cell in multiple myeloma?

Given that multiple myeloma is a monoclonal neoplasm which is originated from plasma cells, there is a controversy whether there exist cancer stem-like cells in MM or not. Could you give me your...

03 April 2018 4,189 0 View

How cancer undergoes "evolution"?

I think that cancer cells undergo "evolutionary dynamics" after chemotherapy and radiation therapy as the selective pressure. Excessive amount of reactive oxygen species (ROS) corresponds to one...

31 December 2017 996 1 View

What is the major pathohistological subtype of lung cancer due to the exposure to multi-walled carbon nanotubes (MWCNTs) in human ?

It has been reported that the animal experiments using rats have revealed the emergence of lung cancer, mainly bronchioalveolar carcinoma and combined carcinoma and adenoma after the exposure to...

31 December 2017 9,084 0 View

How to isolate only endothelial cells of blood vessels by flow cytometry?

When you isolate endothelial cells of the blood vessels, using only CD31 antibody is not enough to isolate them? Why alpha-smooth muscle actin (alpha-SMA) antibody is necessary according to some...

31 December 2017 2,527 2 View

How CD44 and Li-Fraumeni syndrome are related?

We have reported several years ago the 5-year-old male patient with Li-Fraumeni syndrome with osteosarcoma and atypical type of hepatic cancer. Intriguingly, the cancer stem cell marker CD44...

31 December 2017 4,808 2 View

Chemotherapy provokes the ectopic emergence of cancer stem cells in the tumor?

I have reported that chemotherapy for the patient with Li-Fraumeni syndrome results in the ectopic CD44 variant-positive cancer stem cells. Given that chemotherapy often increases the ROS level,...

31 December 2016 6,107 3 View

Denervation is effective for gastric cancer?

Neurotransmitters have been shown to drive tumor progression in the prostate glands and the stomach. In particular, it has recently been shown that acetylcholine promotes proliferation of...

31 December 2016 992 5 View

Do late-competent cancer cells correspond to cancer stem-like cells?

The investigators derived, from a human metastatic lung-cancer cell line and a breast-cancer cell line, latency-competent cells that seed distant sites after experimental tail-vein inoculation...

06 July 2016 8,088 0 View

Expression change in OPA1 and Drp1 is related to the mitochondria dynamics?

While Drp1 contributes to mitochondrial fission, OPA1 has been widely recognized as to promote mitochondrial fusion in cooperative manner with Mfn1/2. Several papers have shown the representative...

05 June 2016 7,579 2 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

No title

02 March 2021 3,060 3 View

How to code open-ended questions in SPSS?

Hello all, In SPSS I am going to code 2 open-ended questions. I have already read all the answers and I made a list of the most important categories to which I can code the answers. This question...

02 March 2021 1,757 4 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View