Is thymic neuroendocrine tumor pathologically and clinically malignant?

Given the recent case report by NEJM (Pallais JC, Fenves AZ, Lu MT, Glomski K. Case 18-2018: A 45-Year-Old Woman with Hypertension, Fatigue, and Altered Mental Status. N Engl J Med....

06 July 2018 1,597 1 View

There is a cancer stem cell in multiple myeloma?

Given that multiple myeloma is a monoclonal neoplasm which is originated from plasma cells, there is a controversy whether there exist cancer stem-like cells in MM or not. Could you give me your...

03 April 2018 4,189 0 View

How cancer undergoes "evolution"?

I think that cancer cells undergo "evolutionary dynamics" after chemotherapy and radiation therapy as the selective pressure. Excessive amount of reactive oxygen species (ROS) corresponds to one...

31 December 2017 996 1 View

What is the major pathohistological subtype of lung cancer due to the exposure to multi-walled carbon nanotubes (MWCNTs) in human ?

It has been reported that the animal experiments using rats have revealed the emergence of lung cancer, mainly bronchioalveolar carcinoma and combined carcinoma and adenoma after the exposure to...

31 December 2017 9,084 0 View

How to isolate only endothelial cells of blood vessels by flow cytometry?

When you isolate endothelial cells of the blood vessels, using only CD31 antibody is not enough to isolate them? Why alpha-smooth muscle actin (alpha-SMA) antibody is necessary according to some...

31 December 2017 2,527 2 View

How CD44 and Li-Fraumeni syndrome are related?

We have reported several years ago the 5-year-old male patient with Li-Fraumeni syndrome with osteosarcoma and atypical type of hepatic cancer. Intriguingly, the cancer stem cell marker CD44...

31 December 2017 4,808 2 View

Chemotherapy provokes the ectopic emergence of cancer stem cells in the tumor?

I have reported that chemotherapy for the patient with Li-Fraumeni syndrome results in the ectopic CD44 variant-positive cancer stem cells. Given that chemotherapy often increases the ROS level,...

31 December 2016 6,107 3 View

Denervation is effective for gastric cancer?

Neurotransmitters have been shown to drive tumor progression in the prostate glands and the stomach. In particular, it has recently been shown that acetylcholine promotes proliferation of...

31 December 2016 992 5 View

Do late-competent cancer cells correspond to cancer stem-like cells?

The investigators derived, from a human metastatic lung-cancer cell line and a breast-cancer cell line, latency-competent cells that seed distant sites after experimental tail-vein inoculation...

06 July 2016 8,088 0 View

Expression change in OPA1 and Drp1 is related to the mitochondria dynamics?

While Drp1 contributes to mitochondrial fission, OPA1 has been widely recognized as to promote mitochondrial fusion in cooperative manner with Mfn1/2. Several papers have shown the representative...

05 June 2016 7,579 2 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View

Is there an appropriate CD8 antibody for IHC-P for staining human breast cancer tissues?

Hello, We are planning on staining Formalin-fixed paraffin breast cancer tissue sections (FFPE) with CD8 Ab via immunofluorescence (Tissue IF). Based on our existing protocol and imaging...

24 February 2021 9,578 4 View

Reagents for histology slides?

Dear all, I am planning to study histology and want to do that at home. I have only stained a blood smear with Giemsa but now want to try a wider range of animal tissues. What reagents will I...

24 February 2021 5,430 3 View