I have recently wondered whether the enhanced SA-beta-gal staining alone really reflects the cellular senescence.
Given the recent case report by NEJM (Pallais JC, Fenves AZ, Lu MT, Glomski K. Case 18-2018: A 45-Year-Old Woman with Hypertension, Fatigue, and Altered Mental Status. N Engl J Med....
06 July 2018 1,803 1 View
Given that multiple myeloma is a monoclonal neoplasm which is originated from plasma cells, there is a controversy whether there exist cancer stem-like cells in MM or not. Could you give me your...
03 April 2018 4,412 0 View
It has been reported that the animal experiments using rats have revealed the emergence of lung cancer, mainly bronchioalveolar carcinoma and combined carcinoma and adenoma after the exposure to...
31 December 2017 9,263 0 View
I think that cancer cells undergo "evolutionary dynamics" after chemotherapy and radiation therapy as the selective pressure. Excessive amount of reactive oxygen species (ROS) corresponds to one...
31 December 2017 1,170 1 View
When you isolate endothelial cells of the blood vessels, using only CD31 antibody is not enough to isolate them? Why alpha-smooth muscle actin (alpha-SMA) antibody is necessary according to some...
31 December 2017 2,711 2 View
We have reported several years ago the 5-year-old male patient with Li-Fraumeni syndrome with osteosarcoma and atypical type of hepatic cancer. Intriguingly, the cancer stem cell marker CD44...
31 December 2017 4,996 2 View
I have reported that chemotherapy for the patient with Li-Fraumeni syndrome results in the ectopic CD44 variant-positive cancer stem cells. Given that chemotherapy often increases the ROS level,...
31 December 2016 6,281 3 View
Neurotransmitters have been shown to drive tumor progression in the prostate glands and the stomach. In particular, it has recently been shown that acetylcholine promotes proliferation of...
31 December 2016 1,171 5 View
The investigators derived, from a human metastatic lung-cancer cell line and a breast-cancer cell line, latency-competent cells that seed distant sites after experimental tail-vein inoculation...
06 July 2016 8,277 0 View
While Drp1 contributes to mitochondrial fission, OPA1 has been widely recognized as to promote mitochondrial fusion in cooperative manner with Mfn1/2. Several papers have shown the representative...
05 June 2016 7,763 2 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
What is the cycling of matter through ecosystems and how energy drives the cycling of matter in photosynthesis and cellular respiration?
02 August 2024 9,518 2 View
Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...
02 August 2024 6,735 1 View
I'm new to primary human fibroblast culture and I'm studying aging using cells from old and young donors. Some research groups have used media supplemented with FGF2, so I cultured cells in the...
29 July 2024 3,030 2 View
Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...
29 July 2024 8,624 1 View
I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...
29 July 2024 6,484 5 View