20 Questions 32 Answers 0 Followers
Questions related from Chinkyu Lee
Hello, today i want to ask about ripa buffer, buffer used in Western blot.(Very simple but seemingly important question) You may already know this, but i found out that there are numerous...
12 October 2017 8,715 3 View
Hello Today i want to get help on how to form graph bar based on qpcr results. I already know how to calculate fold change. For example, Suppose my gene of interest is A and control gene is...
29 April 2017 5,858 2 View
Hello Today i want to ask about whether there is optimal condition of centrifugation (speed, time, and temperature) to obtain cell pellet. I have already searched many protocols, but i only can...
27 March 2017 2,904 4 View
Hello, Today I want to ask about sample buffer in western blot. (For me, I use 4XSB as sample buffer) if sample buffer melts in room temperature, is it okay to use it without any treatments such...
15 November 2016 4,454 3 View
Hello, Today I want to ask whether there is absolute way of extracting proteins from cells for western blot. I found out that there are numerous ways to extract proteins. In my case, I treat...
07 October 2016 3,117 5 View
Hello, Today i want to ask about ratio of developer and fixer in western blot. According to instruction, the dilution ratio of developer and fixer is both 1:4(for example, 200ml developer or...
04 September 2016 5,683 2 View
Hello, I always get great information from researchgate. It seems that i sometimes forget grateful responses to those who replied my questions and I am sorry for that. Today i want to ask optimal...
07 August 2016 1,091 10 View
Hello, Yesterday I asked whether it is ok to analyze migration rates of HUVECs even without previous gelatin coatings during wound healing assays. Of course, I know that gelatin coating should be...
07 May 2016 1,038 2 View
Hello, I want to ask whether gelatin coating is related with migration of HUVECs. I coat gelatin on wells or dishes for well-adherent HUVECs. But In one specific wound healing assay, I cannot...
07 May 2016 1,425 0 View
Hello,I am always cautious on prevention of cell cross contamination.What I express as 'Cross contamination' here is not the mix of cellsof totally different types, but same type of cells treated...
24 April 2016 7,845 8 View
Hello, I asked similar question previously. And I appreciate all of those who answered my question about handling of cDNA. I want to ask 2 questions. 1. Suppose components in mixture are evenly...
19 April 2016 2,521 4 View
Hello, Thanks to RG members who answered my previous question, I could learn that HUVECs do not change to other Cells without special techniques which induce change of cell type during cell...
01 April 2016 1,165 3 View
Hello, Today i want to ask about stability of cDNA. As far as i know, cDNAs are very stable molecules compared to RNAs. During RNA works, I use my personal tools(Pipet tips, 1.5ml tubes, etc) to...
31 March 2016 4,520 7 View
Hello, I always learn a lot thanks to researchgate members. This time I wonder about the cell passage. I often split HUVECs and add passage to splitted HUVECs whenever my splittings are over. But...
20 March 2016 5,444 4 View
Hello, I want to ask very simple and easy questions today. I often conduct siRNA transfection to huvec with clean pippet tips and clean 1.5ml tubes. As far as I know, uses of clean pippet tips and...
17 March 2016 8,289 3 View
Hello, Several days ago I asked about the possibilities of cell change(From HUVECs to other cells) without special methods inducing cell type change. I learned a lot from those who answered that...
17 March 2016 8,127 4 View
Hello, I am an graduate student studying on angiogenesis. I have isolated HUVEs from umbilical vein with collagenase. Here is my question I want to ask. In my protocol, collagenase type 2 is...
25 February 2016 9,436 2 View
Hello I have been working with huvec. When I split huvec cells to new cell dishes, I change pipet aid to prevent trypsin mixing with medium in the medium bottle. But I do not change suction...
06 January 2016 8,274 7 View
Hello! I am just starting research in the filed of vasculogenesis and angiogenesis. I have isolated huvec cells from umbilical vein several times. I learned that during isolation, serum free...
02 January 2016 8,145 9 View
Hello! I am doing research with huvec and I have conducted huvec wound healing assay four times with 6 well plates. I studied that gelatin coating should be done before working with huvec, but it...
25 December 2015 4,185 5 View
