30 Questions 46 Answers 0 Followers
Questions related from Chinkyu Lee
Hello, today i want to ask about ripa buffer, buffer used in Western blot.(Very simple but seemingly important question) You may already know this, but i found out that there are numerous...
12 October 2017 8,761 3 View
Hello I have been getting great support from RG members. Today, i want to ask about phosphorylation. LATS1, which is known to be a critical component of Hippo pathway, regulates YAP. As far as i...
05 August 2017 8,169 2 View
Hello Today i want to get help on how to form graph bar based on qpcr results. I already know how to calculate fold change. For example, Suppose my gene of interest is A and control gene is...
29 April 2017 5,881 2 View
Hello Today i want to ask about whether there is optimal condition of centrifugation (speed, time, and temperature) to obtain cell pellet. I have already searched many protocols, but i only can...
27 March 2017 2,960 4 View
Hello, Today, as described above, I want to ask whether dead cells(Cells dead because of siRNA and DNA cytotoxicity) affect RNA and Protein concentration. I always make sure all samples have same...
24 December 2016 4,014 4 View
Hello, Today I want to ask about sample buffer in western blot. (For me, I use 4XSB as sample buffer) if sample buffer melts in room temperature, is it okay to use it without any treatments such...
15 November 2016 4,482 3 View
Hello, Today I want to ask whether there is absolute way of extracting proteins from cells for western blot. I found out that there are numerous ways to extract proteins. In my case, I treat...
07 October 2016 3,141 5 View
Hello, Today i want to ask about ratio of developer and fixer in western blot. According to instruction, the dilution ratio of developer and fixer is both 1:4(for example, 200ml developer or...
04 September 2016 5,708 2 View
Hello, I always get great information from researchgate. It seems that i sometimes forget grateful responses to those who replied my questions and I am sorry for that. Today i want to ask optimal...
07 August 2016 1,117 10 View
Hello, Yesterday I asked whether it is ok to analyze migration rates of HUVECs even without previous gelatin coatings during wound healing assays. Of course, I know that gelatin coating should be...
07 May 2016 1,065 2 View
Hello, I want to ask whether gelatin coating is related with migration of HUVECs. I coat gelatin on wells or dishes for well-adherent HUVECs. But In one specific wound healing assay, I cannot...
07 May 2016 1,444 0 View
Hello,I am always cautious on prevention of cell cross contamination.What I express as 'Cross contamination' here is not the mix of cellsof totally different types, but same type of cells treated...
24 April 2016 7,872 8 View
Hello, I asked similar question previously. And I appreciate all of those who answered my question about handling of cDNA. I want to ask 2 questions. 1. Suppose components in mixture are evenly...
19 April 2016 2,539 4 View
Hello, This time I want to ask about the ways of labeling human genes. As fas as i know, human gene is written with big alphabets.(For example, If the name of my human gene of interest is ABC, the...
03 April 2016 8,320 6 View
Hello, This time I want to ask about Wound healing assay. To analyze angiogenic behaviors of HUVECs, I conducted wound healing assay with HUVECs many times and will start beads sprouting assay...
02 April 2016 5,379 3 View
Hello, Thanks to RG members who answered my previous question, I could learn that HUVECs do not change to other Cells without special techniques which induce change of cell type during cell...
01 April 2016 1,187 3 View
Hello, Today i want to ask about stability of cDNA. As far as i know, cDNAs are very stable molecules compared to RNAs. During RNA works, I use my personal tools(Pipet tips, 1.5ml tubes, etc) to...
31 March 2016 4,539 7 View
Hello, I always learn a lot thanks to researchgate members. This time I wonder about the cell passage. I often split HUVECs and add passage to splitted HUVECs whenever my splittings are over. But...
20 March 2016 5,477 4 View
Hello, I want to ask very simple and easy questions today. I often conduct siRNA transfection to huvec with clean pippet tips and clean 1.5ml tubes. As far as I know, uses of clean pippet tips and...
17 March 2016 8,313 3 View
Hello, Several days ago I asked about the possibilities of cell change(From HUVECs to other cells) without special methods inducing cell type change. I learned a lot from those who answered that...
17 March 2016 8,157 4 View
Hello, These days I often ask various questions in researchgate quite often. Previously, I asked about the addition of antibiotics(penicillin / streptomycin) in media. I got answers from four...
15 March 2016 1,930 2 View
Hello, I always get great help from researchgate. Today I want to ask about cell culture medium. I work with huvec, studying angiogenesis. In general, I use m199 for working with huvec but...
14 March 2016 9,661 4 View
Hello, Today I want to ask about change of cell type. Before asking, I want to deeply appreciate those who answered my previous question about mRNAs as cell markers. Here is my question : I am...
08 March 2016 931 8 View
Hello, I am an graduate student studying on angiogenesis. I have isolated HUVEs from umbilical vein with collagenase. Here is my question I want to ask. In my protocol, collagenase type 2 is...
25 February 2016 9,451 2 View
Hello, I am an graduate student who work with huvec. Some days ago, I did wound healing assay with HUVECs. After culturing the cells with 20% EGM, I starved HUVECs with approximately 1% EBM(50ml...
24 February 2016 3,471 3 View
Hello,I want to ask one question about wound healing assay.I use pippet tip to make a wound.If i use same pippet tip to make all wounds in huvec monolayer of different 6 wells,I think that huvec...
25 January 2016 7,767 0 View
Hello, I want to ask one question, and I know it may be too basic for experienced and advanced researchers. To work with huvec, I preheat 0% m199, 20% m199 , and Trypsin. I also use opti-mem /...
20 January 2016 3,781 2 View
Hello I have been working with huvec. When I split huvec cells to new cell dishes, I change pipet aid to prevent trypsin mixing with medium in the medium bottle. But I do not change suction...
06 January 2016 8,296 7 View
Hello! I am just starting research in the filed of vasculogenesis and angiogenesis. I have isolated huvec cells from umbilical vein several times. I learned that during isolation, serum free...
02 January 2016 8,178 9 View
Hello! I am doing research with huvec and I have conducted huvec wound healing assay four times with 6 well plates. I studied that gelatin coating should be done before working with huvec, but it...
25 December 2015 4,206 5 View
