Hello,
Yesterday I asked whether it is ok to analyze migration rates of HUVECs
even without previous gelatin coatings during wound healing assays.
Of course, I know that gelatin coating should be conducted.
I attached one file from one of my HUVEC pictures.
From the picture, HUVECs seem to adhere to bottom quite well.
But it is just my opinion so I want to ask whether from this picture,
there is a way to figure out that I coated gelatin on the bottom of cell dishes.
The assay was conducted several months ago, so I cannot remember
whether I coated gelatin.
Thank you very much!
The National Institute of Health (NIH) image processing software ImageJ ( https://imagej.nih.gov/ij/ ) is commonly used to measure cell size, track cell movement and other behavior. One necessary piece of information to do any measurements is a calibration mark on the original photo. Once you add the calibration mark, you can measure the size of the gap, count the cells, measure the size of the cells and other techniques. Many ImageJ tutorials are available online.
If you still have the plates, you could try staining a well with Ponceau S dye. If you first coated with gelatin, the scratch will be red.
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