Hello,
Today i want to ask about ratio of developer and fixer in western blot.
According to instruction, the dilution ratio of developer and fixer is both
1:4(for example, 200ml developer or fixer and 800ml water)
But i recognized that ratio can be different than 1:4 due to my error
during dilution. But I have found no problem considering my detection results in western blot.
So I concluded that the error in dilution ratio is just minute
and is not critical in my detection results.
But it is just my conclusion so I want to get more objective feedback
from others. Thank you!
Hey!! I was always using developer and fixer at a 1:5 dilution and I had pretty good results
The dilution ratio is not so crucial for developer and fixer. It is OK for just weak variation of dilution ratio. Acturally, the time and temperature are more important for the reaction.
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