No title

31 December 2019 8,862 3 View

Which citation style include almost every information. like publishing house for the journal too?

Which citation style include almost every information i.e. most detailed including every information on the journal like publishing house for the journal too?

10 November 2019 1,532 3 View

What's the difference between TOP10 and JM109 Ecoli strains?

Whats the difference between TOP10 and JM109 Ecoli strains? Is that the TOP10 able to express GFP tagged protein and can be visualized under blue light if we are doing cloning, then what makes it...

09 October 2019 3,378 3 View

What would be life time difference between soluble protein and amyloids?

How would be amyloid and soluble protein life time difference? if we have cells in which we tagged protein with eGFP and then FLIM image them and compare within cell the life time. How would it be...

08 September 2019 9,659 1 View

FLIM data analysis?

I got a ".lif" file from confocal FLIM imaging, I have 0 min and 60 min data with 15 min interval. I want to show lifetime for these timepoints. How can I start with analysis and making figures...

08 September 2019 2,123 1 View

Would ZnF motif (in my protein of interest) get destroyed by TCEP during labeling of Fluorescent dye to Cysteine in protein?

My protein has ZnF motif, would using of TCEP destroy this and have effect on RNA binding as I need to use it for the Cys labelling with fluorescent dye.

02 March 2019 3,801 4 View

Should I add PI, PMSF and Peptstatin during protein purification in all buffers?

Should I add PI, PMSF and Peptstatin during protein purification in all buffers like resuspension, wash elution buffer in all steps or just until first wash buffer and second wash buffer as well...

31 December 2018 2,155 11 View

mEOS3 tagged protein concentration by spectrophotometer?

I have protein tagged with mEOS3 and want to check it's concentration after purification with spectrophotometer. How can I do that? What wavelength I should use and what Extinction coefficient to...

31 December 2018 8,749 2 View

No title

10 November 2018 9,291 3 View

What does tis sentence mean?

The abc (region of a protein) of xyz (protein name) binds the 50kDa ZYX (protein name) with dissociation constant (kDa) of 30nM. Can one explain what does this dissociation constant mean and what...

08 September 2018 768 1 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

Does anyone know how to plot my station to a section plot on Ocean Data View (ODV)?

I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...

01 March 2021 3,610 1 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

How can I make stacked bar graph with scatter plot?

I want to make a stacked bar graph with showing each data value as dots. I tried with graph pad prism, version 6, but could not succeed. Does anyone have tried before making such graphs?

01 March 2021 9,383 1 View

How to assign different material properties to each element in Abaqus ?

Hello, I am writing a python script to generate my model and write the input file. The problem is I have 1000 elements in my mesh and I want to assign different material properties to each...

01 March 2021 9,511 3 View

What could be reason for getting such type of graph for complex impedance in case of polymer blend films?

Its effect appears in real part of ac conductivity also. Can anyone suggest any reason behind it?

26 February 2021 3,487 3 View

Can I use a graph from a another source in a thesis?

I have a permission from X company to use their graph from a report, but they have a reference in the bottom left corner of the graph as follows: "Source: Database Y". Is it OK to use this graph...

24 February 2021 2,269 3 View

I have need of XRD graph analysis software for phase identification. Can you please provide links for software tools which can be downloaded?

i am trying to download x'pert high score software, but can't download it. please do suggest software for phase analysis

24 February 2021 5,687 4 View

Could you please suggest to me research in which a relation between the shear wave velocity and the degree of saturation for stone was discussed?

Hi all, I am looking for a graph or a relation that relates the degree of saturation and the shear wave velocity for the marlstone. Looking forward to your suggestions Best Regards

24 February 2021 9,690 1 View

Which microtomy technique do you suggest for thin and delicate root sectioning ?And, how to preserve and process the roots before sectioning?

We have thin roots of from 1 year old different species of plants. We are looking into anatomical details of the roots. What's the best method to preserve and process the roots once they are...

23 February 2021 1,898 1 View