No title

31 December 2019 8,862 3 View

Which citation style include almost every information. like publishing house for the journal too?

Which citation style include almost every information i.e. most detailed including every information on the journal like publishing house for the journal too?

10 November 2019 1,532 3 View

What's the difference between TOP10 and JM109 Ecoli strains?

Whats the difference between TOP10 and JM109 Ecoli strains? Is that the TOP10 able to express GFP tagged protein and can be visualized under blue light if we are doing cloning, then what makes it...

09 October 2019 3,378 3 View

What would be life time difference between soluble protein and amyloids?

How would be amyloid and soluble protein life time difference? if we have cells in which we tagged protein with eGFP and then FLIM image them and compare within cell the life time. How would it be...

08 September 2019 9,659 1 View

FLIM data analysis?

I got a ".lif" file from confocal FLIM imaging, I have 0 min and 60 min data with 15 min interval. I want to show lifetime for these timepoints. How can I start with analysis and making figures...

08 September 2019 2,123 1 View

Would ZnF motif (in my protein of interest) get destroyed by TCEP during labeling of Fluorescent dye to Cysteine in protein?

My protein has ZnF motif, would using of TCEP destroy this and have effect on RNA binding as I need to use it for the Cys labelling with fluorescent dye.

02 March 2019 3,801 4 View

Should I add PI, PMSF and Peptstatin during protein purification in all buffers?

Should I add PI, PMSF and Peptstatin during protein purification in all buffers like resuspension, wash elution buffer in all steps or just until first wash buffer and second wash buffer as well...

31 December 2018 2,155 11 View

mEOS3 tagged protein concentration by spectrophotometer?

I have protein tagged with mEOS3 and want to check it's concentration after purification with spectrophotometer. How can I do that? What wavelength I should use and what Extinction coefficient to...

31 December 2018 8,749 2 View

No title

10 November 2018 9,291 3 View

What does tis sentence mean?

The abc (region of a protein) of xyz (protein name) binds the 50kDa ZYX (protein name) with dissociation constant (kDa) of 30nM. Can one explain what does this dissociation constant mean and what...

08 September 2018 768 1 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Image Processing and classification using keras/Tensorflow?

Do you have some recommendations approaches on Image processing and classification of plant diseases using TensorFlow/Keras?

02 March 2021 1,493 4 View

Which SAR dataset to use for generating coherency matrix and performing target decomposition (Description) ?

Working on chandrayaan-2 DFSAR data, there are three datasets available: 1) Slant range image data product: The slant range complex image file. Each pixel is represented by two 4-byte floating...

02 March 2021 8,481 3 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

Quantifying leaking DNA from bacteria - what's the secret?

Hi, all! I need to determine if my treatment causes membrane damage in bacteria (S. aureus and E. cloacae), but I cannot use a fluorescent probe such as PI and similar ones because my compound is...

24 February 2021 2,006 2 View

Where can find Medical Image Data Sets for Machine learning research project?

I want to work on medical image(disease) detection and classification. Where can I found Image data sets?

24 February 2021 3,130 3 View

IF troubleshooting: looking at an intracellular protein that localizes to the cell's and how to reduce increase in signal due to cell ruffles?

I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...

24 February 2021 8,582 1 View