No title

31 December 2019 8,862 3 View

Which citation style include almost every information. like publishing house for the journal too?

Which citation style include almost every information i.e. most detailed including every information on the journal like publishing house for the journal too?

10 November 2019 1,532 3 View

What's the difference between TOP10 and JM109 Ecoli strains?

Whats the difference between TOP10 and JM109 Ecoli strains? Is that the TOP10 able to express GFP tagged protein and can be visualized under blue light if we are doing cloning, then what makes it...

09 October 2019 3,378 3 View

What would be life time difference between soluble protein and amyloids?

How would be amyloid and soluble protein life time difference? if we have cells in which we tagged protein with eGFP and then FLIM image them and compare within cell the life time. How would it be...

08 September 2019 9,659 1 View

FLIM data analysis?

I got a ".lif" file from confocal FLIM imaging, I have 0 min and 60 min data with 15 min interval. I want to show lifetime for these timepoints. How can I start with analysis and making figures...

08 September 2019 2,123 1 View

Would ZnF motif (in my protein of interest) get destroyed by TCEP during labeling of Fluorescent dye to Cysteine in protein?

My protein has ZnF motif, would using of TCEP destroy this and have effect on RNA binding as I need to use it for the Cys labelling with fluorescent dye.

02 March 2019 3,801 4 View

Should I add PI, PMSF and Peptstatin during protein purification in all buffers?

Should I add PI, PMSF and Peptstatin during protein purification in all buffers like resuspension, wash elution buffer in all steps or just until first wash buffer and second wash buffer as well...

31 December 2018 2,155 11 View

mEOS3 tagged protein concentration by spectrophotometer?

I have protein tagged with mEOS3 and want to check it's concentration after purification with spectrophotometer. How can I do that? What wavelength I should use and what Extinction coefficient to...

31 December 2018 8,749 2 View

No title

10 November 2018 9,291 3 View

What does tis sentence mean?

The abc (region of a protein) of xyz (protein name) binds the 50kDa ZYX (protein name) with dissociation constant (kDa) of 30nM. Can one explain what does this dissociation constant mean and what...

08 September 2018 768 1 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

How to remove the residual PDMS on the silicon mold?

Incompletely cross-linked pdms are difficult to remove.

01 March 2021 798 3 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

What is the most suitable medium of ascomycetes fungi ??

What is the most suitable medium of ascomycetes fungi ??

26 February 2021 4,413 1 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Can anyone help me how to get the similar bands in Actin?

I run western blot on HCT-116 cell lines, I got actin band like in the attached picture. The band size is not same. Can anyone help me explain this situation and how to overcome? Thank you your...

22 February 2021 6,937 3 View

How we can measure Kemp % in wool without using of benzene?

Would you please let me to know about the methods for measurement of medullation and Kemp % on animal? My problem is that the wool sampling and sending to laboratory is not possible. So I would...

21 February 2021 7,260 4 View

Hi, can anybody help me with the suggestions how can I amplify a stress induced gene of 1107 bp?

I am trying to amplify the TGA1 (Transcription factor 1 ) of Arabidopsis ,which is 1107 bp. I have treated the plants with Salycylic solution over night and extracted the RNA and make cDNA. I...

17 February 2021 7,802 2 View

Can 5-FOA Diffuse Through Agar?

I am preparing to do a gene knockout in the filamentous fungi Coccidioides posadasii targeting pyrG/URA3 so that 5-FOA can be used for selection pressure instead of traditional antibiotic...

16 February 2021 169 4 View

Any specific reason, how sclerotium rolfsii can grow out of Petridish?

Is it related to nutrition or any other reason? Any other fungus have similar kind of growth pattern?

09 February 2021 4,357 4 View