Yesterday I found something strange in one of our cell cultures.. Maybe someone can recognise this and could tell what it is? What is this? :)
I think that it was at x20 (10x20) but I am not sure for now. I took this photo with my smartphone by applying it to the ocular of Olympus XT 81.
EDIT: It was taken at x10 for sure.
Nope.. that is some garbage and mayby fat drops (we isolated ADSC from fat tissue). In the whole dish there were no cells except this "things".
Hi Bartosz,
Can you give more Information about cultivation used (medium, which cells ?)
Is it only this one or do other cells look the same.
It might be the formation of stress filopodia. This could make sense if the size of your "discovery" fits to the normal size of your cells.
Good luck
Cheers Tobias
We isolated ADSC from fat tissue. We applied our standard method with 2hours of digestion with colagenase which usually worked. The medium was DMEM with 10%fbs with addidion of antibioticts.
In all dishes we obtained our goal and have standard cultures of these cells, but in this one dish there was nothing except this structures. There were about 10-20 cells like this in whole dish. The size of this structures is similar to normal cells that we expected.
I agree with Tobias. I have seen structures like these before in stem cell cultures.
These cells have been differentiated into neuronal like cells. Means the cells has lost it capacity to divide further
Anjali are you sure about this? That was one of my thoughts.. That is interesting. Mayby nothing has differentiated, i think that it could be the neuronal cell somehow isolated straight from the tissue :) I don't have any experience with neuronal cells. Yup, it looks like neuronal.. thanks for the answer too.
In my humble opinion, this is a bi-nucleated cell (the nuclei are in the upper half), the origin of the protrusions cannot be determined easily from the photo*. To be sure about the cell identity, try, e.g., some immunofluorescent staining for adipocyte marker. Furthermore, compare this cell with the others in the culture and search in the literature.
*A colleague of mine observed this morphology in transfected cells which resulted in an impaired cell division the cells multiplicated but the the cytokinesis didn't occurred. Additionally, some cells (such as hepatocytes) are naturally multinucleated.
You can read more on neuronal cells and go for nestin icc a marker for neuronal cells. But the culture characteristics are similar to neuronal cells
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