Why is TMB (HRP substrate) not giving the expected color?

Hello all. We are trying to prepare TMB (HRP substrate) in house. 1 mg TMB (biobasic) was dissolved in 10 mL phosphate-citrate buffer (pH 5.0). Two uL H2O2 was added and an aliquot of complete...

29 October 2015 8,565 16 View