8 Questions 26 Answers 0 Followers
Questions related from Tomas Machacek
Has anybody ever tried to perform antigen retrieval on frozen tissue sections by incubation in sucrose? We tried that in case of murine iNOS in the spinal cord and it works pretty nice. However, I...
29 May 2019 829 3 View
I want to induce nitric oxide (NO) production in mice by LPS i.p. administration and then measure nitrite/nitrate concentration in serum. However, there is a huge variation of LPS concentrations...
15 September 2017 1,457 1 View
I would like to prevent iNOS activity in mice. Based on literature, I chose aminoguanidine as the inhibitor to be administered p.o. in drinking water. Which of these two compounds...
20 October 2015 9,747 2 View
Personally, I am convinced this happens (based on my own 3-year experience with the same parasite strain) but I haven't found it in literature for any helminth.
21 April 2015 7,625 3 View
If you need to label living schistosome cercariae to track them intravitaly, what stain would you use?
16 April 2015 2,696 6 View
Using the antibody linked in the end, I still get at least three non-specific bands in astrocyte lysates. Does anybody have the same problem? http://www.sigmaaldrich.com/catalog/product/sigma/n9657
04 February 2015 8,128 3 View
I haven't used it and seen it in papers very often but my colleague uses it in case of macrophages. So I am just curious about your opinion/experience.
13 August 2014 5,836 2 View
I have tried to isolate murine microglial cells from adults' spinal cords and brains using the protocol by Cardona et al. (2006) [http://www.ncbi.nlm.nih.gov/pubmed/17487181]. However, I failed -...
13 February 2014 7,831 35 View
