Tomas Machacek

5 Questions 22 Answers 0 Followers

Questions related from Tomas Machacek

Antigen retrieval in IHC - any experience with sucrose?

Has anybody ever tried to perform antigen retrieval on frozen tissue sections by incubation in sucrose? We tried that in case of murine iNOS in the spinal cord and it works pretty nice. However, I...

29 May 2019 745 3 View

Which iNOS inhibitor should I choose to treat mice and ducks?

I would like to prevent iNOS activity in mice. Based on literature, I chose aminoguanidine as the inhibitor to be administered p.o. in drinking water. Which of these two compounds...

20 October 2015 9,670 2 View

Do you know any intravital fluorescent staining available for schistosome cercariae?

If you need to label living schistosome cercariae to track them intravitaly, what stain would you use?

16 April 2015 2,634 6 View

Is anybody experienced with this anti-iNOS antibody (Sigma) in Western blot assays?

Using the antibody linked in the end, I still get at least three non-specific bands in astrocyte lysates. Does anybody have the same problem? http://www.sigmaaldrich.com/catalog/product/sigma/n9657

04 February 2015 8,056 3 View

Microglia isolation using Percoll gradient?

I have tried to isolate murine microglial cells from adults' spinal cords and brains using the protocol by Cardona et al. (2006) [http://www.ncbi.nlm.nih.gov/pubmed/17487181]. However, I failed -...

13 February 2014 7,744 35 View