hello!

I am trying to use a yeast display technology to screen a 120kDa protein of interest. To this end I cloned my protein of interest into the pYD1 plasmid and sequence verified the construct (it is in frame with the Xpress epitope). I used 2% galactose to induce the yeast for 24h at 20C. Unfortunately I do not see any expression of my POI when screening the yeast flow cytometry. However, I do get expression of the Xpress epitope so the induction must have worked as the yeast is something. Any thoughts and troubleshooting advice would be greatly appreciated! :)