3 Questions 3 Answers 0 Followers
Questions related from Anna Meister
hello! I am trying to use a yeast display technology to screen a 120kDa protein of interest. To this end I cloned my protein of interest into the pYD1 plasmid and sequence verified the construct...
18 November 2024 385 0 View
Hello, I am currently trying to optimise a bacterial expression system using BL2 1DE3 bacteria and IPTG. In order to just have a quick look, whether I can see any protein overexpression on a...
05 May 2024 3,610 2 View
Hello! I have been trying to purify an antibody using protein A column chromatography but noticed that as soon as the antibody equate hits the neutalisation buffer (Tris, NaOH, ph 9) it forms a...
23 October 2023 9,869 5 View