I am trying to develop subcutaneous xenograft model in NUDE mice, using DND-41 tumor cells. I already tried sometimes, without success. I am using 100% and/or 50% of matrigel.
Someone knows if is mandatory a special immunodeficient mice for this kind of cells?
Thank you!
May be try more cells, more mice and wait longer. Matrigel have to help
Jian Zuo, I am not using NOD/SCID. I am using a immunodeficient mice, but they still have NK cells and macrophages. I found a paper that used this cell DND-41 in Nu/Nu CD1 NUDE, but I don't know why this strain could be better than mine NUDE.
Hi Karina Spunde, I didn`t test my cells with soft agar. But why it can be better than matrigel?
Soft agar is in vitro test for anchorage indepedent ability to grow.
In your case, I think you have to work to get tumour in mice and then, isolate from it the cells, which are able to grow on nude mice. Not all cells in your culture have such a capability, so you have to work to select such cells from your cell culture and to establish such a subline.
Use at least 10 to 7 and more cells with matrigel and wait at least 4 weeks. You can try also to ask from the authors of paper the cells wich already are able to grow in nude mice.
Techologically, CD-1 nu/nu mice have same immunodeficient level with nude mice. Actully, CD-1 nu/nu mice is one of nude mice also. I don't know the mice background you used. I assume it maybe Balb/c nude mice? I don't think there is too much difference between these two strain. But, rarely, some cell line maybe need special genetical background, ie, CD-1.
But, mostlikely, the more severe immunodeficent mice should be more easy for tumoe development. That's why I suggest you to try Scid or Nod-Scid mice.
Nude mice have high NK levels which reject all but the fastest growing tumor cellls, Jackson labs' NSG mice are the most immune deficient
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