Would you like to share your experience on breast cancer stem cells sorting from mammosphere by using MACS column free methods?

More Ming Chuan Yu's questions See All

Can anyone give web links and published e-books and papers?

Can anyone give web links and published e-books and papers telling about why anti-tumor drugs killed cancer cells first before normal cells/stem cells? Generally it must be related to metabolic...

01 February 2018 8,250 0 View

Has any one seen writing 10 power 4 as 10^4 or 10e4 in published paper?

writing 10 power 4 such as 10^4 or 10e4 rather than making 4 as super script in publish papers. Can let let me have links for such papers if you have seen. Thanks.

31 December 2017 3,191 0 View

How to make serum free freezing medium?

I was looking for ingredients of serum free freezing medium but I cannot find anywhere or its discussion in this research gate. So Can anyone share your knowledege on making serum free freezing...

09 October 2017 9,271 5 View

Instead of neutralizing trypsin by FBS, how do you think if we can just wash them out with PBS?

I like to know whether it is possible just to wash out trypsin with PBS, centrifuge followed by resuspending the pellet with serum free medium for cells to be cultured with serum free medium?

08 September 2017 4,327 6 View

Has anyone here experience with culturing Breast cancer stem cells (Parental)?

I would like to know your experience on culturing Human breast cancer stem cells (Parental) Thanks.

07 August 2017 7,330 4 View

Is any one here using Biolog PMM for mammalian cells phenotyping?

I would like to get some practical info with regard to biolog PMM phenotyping. I also have done some and would like to share info too.

06 July 2017 1,282 2 View

Do we need to do statistical analysis for miRNA expression that showed in fold change?

I get fold change of miRNAs expression after running qPCR. I would like to know whether I need to do statistical analysis for those fold change or not? in fold change, if expression level is above...

05 June 2017 5,018 5 View

What is the best way to prove primary breast cancer epithelial cells in cultured flasks are tumor cells?

I am currently culturing primary breast cancer epithelial cells from human breast cancer patient. It has been about 3 to 4 months and now epithelial cells are growing well and no more fibroblast...

09 October 2016 3,084 4 View

What are the Q1, Q2, Q3 or Tier 1, 2, 3 journals in life science?

How should we look for list of journals of Q1, Q2, Q3 respectively? I cannot find any proper list. Some said the higher the impact factor, the higher the tier is and other said no, it is not as...

07 August 2016 2,529 0 View

How to remove debris after tissue dissociation for stem cell culture?

I am facing an issue of debris after dissociation. These debris are mixed with dissociated cells. As I am using serum free and ultra low attachment flask, it is really hard to remove them. If you...

06 July 2016 8,998 2 View

What are the emerging trends and opportunities for STEM education and careers in the online space?

10 August 2024 507 0 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View