Can anyone give web links and published e-books and papers?

Can anyone give web links and published e-books and papers telling about why anti-tumor drugs killed cancer cells first before normal cells/stem cells? Generally it must be related to metabolic...

01 February 2018 8,093 0 View

How to make serum free freezing medium?

09 October 2017 9,085 5 View

Instead of neutralizing trypsin by FBS, how do you think if we can just wash them out with PBS?

08 September 2017 4,154 6 View

Has anyone here experience with culturing Breast cancer stem cells (Parental)?

07 August 2017 7,179 4 View

Is any one here using Biolog PMM for mammalian cells phenotyping?

06 July 2017 1,123 2 View

Do we need to do statistical analysis for miRNA expression that showed in fold change?

I get fold change of miRNAs expression after running qPCR. I would like to know whether I need to do statistical analysis for those fold change or not? in fold change, if expression level is above...

05 June 2017 4,877 5 View

What is the best way to prove primary breast cancer epithelial cells in cultured flasks are tumor cells?

I am currently culturing primary breast cancer epithelial cells from human breast cancer patient. It has been about 3 to 4 months and now epithelial cells are growing well and no more fibroblast...

09 October 2016 2,887 4 View

What are the Q1, Q2, Q3 or Tier 1, 2, 3 journals in life science?

How should we look for list of journals of Q1, Q2, Q3 respectively? I cannot find any proper list. Some said the higher the impact factor, the higher the tier is and other said no, it is not as...

07 August 2016 2,338 0 View

What is the ideal wave length to apply for MTT assay plate reading?

I would like to add one query here about plate reading wave length. It is very confusing as some use 570 and ref 630, some other use 540 and ref 720, and also found 570 but no reference. So what...

06 July 2016 715 6 View

How to remove debris after tissue dissociation for stem cell culture?

I am facing an issue of debris after dissociation. These debris are mixed with dissociated cells. As I am using serum free and ultra low attachment flask, it is really hard to remove them. If you...

06 July 2016 8,818 2 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

How to write a scientific report?

We are analysing scientific reports at University in great detail, so I wanted to find a scientific report about how to write a scientific report. Including detail such as what information to put...

02 March 2021 7,602 4 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View

Energy minimization in LAMMPS using NVT ?

How do I do energy minimization in lammps with NVT ensemble ? I am using the following command and it does not seem to work. It stops at extremely high energy and gives me the error zero search...

01 March 2021 2,294 3 View

No title

01 March 2021 3,905 5 View

How to Balance Bench work with Reading and Writing?

I am a second-year grad student. I am finding it difficult finding time to read literature and write (things like qualifying exam, thesis, grants). I find myself so busy with bench work that I...

01 March 2021 3,325 4 View

How to modify Johnson cook equation in ABAQUS?

I wanted to add an extra parameter to the existing Johnson cook equation in the ABAQUS simulation. How to add those extra parameters. Ref: A modified Johnson-Cook material model with strain...

28 February 2021 4,841 3 View

Which software is better to write systematic review paper?

We have seen some authors use some software to write review paper. As obvious, the study of all published papers concerning our studying field is not possible and these software can help us to...

27 February 2021 6,296 1 View