What could be the reason of no transformation using a ligation mixture?

Recently, I have been working with some genes contained in pBlueScript II SK (+) and that contain XhoI and XbaI restriction sites. I would like to clone them in PET28a. For instance, the...

22 June 2020 3,474 5 View

What factors influence user acceptance of affective technologies?

Affective technologies are the interfaces concerning the emotional artificial intelligence branch known as affective computing (Picard, 1997). Applications such as facial emotion recognition...

08 May 2020 2,973 9 View

Are there zinc-CYS parameters in CHARMM files? of has anyone generated these parameters?

06 March 2020 5,939 2 View

How do I run a successful digestion for a large plasmid with GC Repeats, hairpins, and potentially other secondary structure?

I am doing some cloning and am having some issues with both sequencing my 22kb plasmid and running digestions with it. I sent my plasmid in for sequencing and got terrible results, but...

02 July 2019 1,506 3 View

How to assess machine learning tools in eCognition Developer version 9.3.2?

How to assess machine learning classification tools in the latest release of eCognition Developer 9.3.2.

17 September 2018 8,963 1 View

I am looking for a simple basic description of a system within systems theory used to describe an organisation?

I have studied systems theory in the more complex mathematical version and have a handout in Dutch describing an organisation as a system with the primary process and two kinds of feedback loops....

15 April 2018 6,746 2 View

Preparation of spheroids for microscopic analysis

I'm using HepG2 to form 3D HepG2 aggregates with alginate, to confocal microscopy analyzes. However, I have difficulty to maintaining the spheroids after fixation and all procedures of incubations...

24 July 2017 478 1 View

Does anyone work with esophageal cell line immortalized with hTERT ?

22 June 2016 3,575 3 View

Why does an H&E stained slide that showed the presence of calcium does not stain positive for von Kossa?

22 February 2015 9,571 4 View

ERK5 Ab to ChiP:

Does anyone use Anti ERK5 to Chromatin Immunoprecipitation assay (ChiP)? I want to start this protocol but dont know where I can find this Ab.

14 May 2013 2,365 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

Do you kow of any database containing data on retention time, log P and polarizability for a large number of molecules?

Hi, I am a chemistry undergraduate doing my dissertation project on HPLC. In my project, I need to predict the HPLC retention time of 20 aromatic molecules, the calibration set. To do this I will...

24 February 2021 2,602 1 View

How to seperate PEG-4000 from aqueuse phase ?

Hello everyone , Please tell me if It possible to seperat POlyethelyn glycol -4000 from an aqueuse media by other ways rather heating . I have tried salting out by electrolyte solution to...

22 February 2021 7,285 5 View

How to analyse monosaccharide compositions from soluble fiber?

I have tried to hydrolyze the sample using 2 M TFA at 100C for 24, 48 h and 120C for 2 h. From these conditions, the HPLC results showed that peaks were not separated into monosaccharide. I found...

17 February 2021 5,230 1 View

No title

Hello. I ask for help - I need to get lithium tetraborate (soluble salt) from lithium carbonate and boric acid. In literature, everything seems to be simple - mix, ignite and - it's done. But it...

16 February 2021 9,523 2 View

Is There are any idea to achieve H NMR signals for swollen compound ??

the samples are not completely soluble - just swelled the solvents that's mean the sample may be partially soluble any suggestion, please

16 February 2021 8,801 3 View

Which non-coordinating solvent dissolves anhydrous Iron(II)chloride?

Hallo, i am looking for a non coordinating solvent that dissolves anhydrous FeCl2 for a synthesis in which i can not use solvents like thf, pyridin etc.. I already tried benzene (no solubility),...

11 February 2021 6,105 1 View

DMSO concentration in cell culture? Precipitating while PBS is added?

I am testing a compound on RAW 264.7. the compound is soluble in DMSO. but to ensure that final concentration of dmso is not more than 0.1%, we are first dissolving it in dmso and then diluting it...

11 February 2021 1,136 3 View

Solubility of PMP in solvent?

What is the maximum solubility limit of poly(4-methyl-1-pentene) in N,N, dimethylformamide? I gone through solubility parameter concept but I did not get the answer for maximum amount of polymer...

02 February 2021 947 3 View