What could be the reason of no transformation using a ligation mixture?

Recently, I have been working with some genes contained in pBlueScript II SK (+) and that contain XhoI and XbaI restriction sites. I would like to clone them in PET28a. For instance, the...

22 June 2020 3,474 5 View

What factors influence user acceptance of affective technologies?

Affective technologies are the interfaces concerning the emotional artificial intelligence branch known as affective computing (Picard, 1997). Applications such as facial emotion recognition...

08 May 2020 2,973 9 View

Are there zinc-CYS parameters in CHARMM files? of has anyone generated these parameters?

06 March 2020 5,939 2 View

How do I run a successful digestion for a large plasmid with GC Repeats, hairpins, and potentially other secondary structure?

I am doing some cloning and am having some issues with both sequencing my 22kb plasmid and running digestions with it. I sent my plasmid in for sequencing and got terrible results, but...

02 July 2019 1,506 3 View

How to assess machine learning tools in eCognition Developer version 9.3.2?

How to assess machine learning classification tools in the latest release of eCognition Developer 9.3.2.

17 September 2018 8,963 1 View

I am looking for a simple basic description of a system within systems theory used to describe an organisation?

I have studied systems theory in the more complex mathematical version and have a handout in Dutch describing an organisation as a system with the primary process and two kinds of feedback loops....

15 April 2018 6,746 2 View

Preparation of spheroids for microscopic analysis

I'm using HepG2 to form 3D HepG2 aggregates with alginate, to confocal microscopy analyzes. However, I have difficulty to maintaining the spheroids after fixation and all procedures of incubations...

24 July 2017 478 1 View

Does anyone work with esophageal cell line immortalized with hTERT ?

22 June 2016 3,575 3 View

Why does an H&E stained slide that showed the presence of calcium does not stain positive for von Kossa?

22 February 2015 9,571 4 View

ERK5 Ab to ChiP:

Does anyone use Anti ERK5 to Chromatin Immunoprecipitation assay (ChiP)? I want to start this protocol but dont know where I can find this Ab.

14 May 2013 2,365 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

How to remove the residual PDMS on the silicon mold?

Incompletely cross-linked pdms are difficult to remove.

01 March 2021 798 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

What is the most suitable medium of ascomycetes fungi ??

What is the most suitable medium of ascomycetes fungi ??

26 February 2021 4,413 1 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View