What could be the reason of no transformation using a ligation mixture?

Recently, I have been working with some genes contained in pBlueScript II SK (+) and that contain XhoI and XbaI restriction sites. I would like to clone them in PET28a. For instance, the...

22 June 2020 3,474 5 View

What factors influence user acceptance of affective technologies?

Affective technologies are the interfaces concerning the emotional artificial intelligence branch known as affective computing (Picard, 1997). Applications such as facial emotion recognition...

08 May 2020 2,973 9 View

Are there zinc-CYS parameters in CHARMM files? of has anyone generated these parameters?

06 March 2020 5,939 2 View

How do I run a successful digestion for a large plasmid with GC Repeats, hairpins, and potentially other secondary structure?

I am doing some cloning and am having some issues with both sequencing my 22kb plasmid and running digestions with it. I sent my plasmid in for sequencing and got terrible results, but...

02 July 2019 1,506 3 View

How to assess machine learning tools in eCognition Developer version 9.3.2?

How to assess machine learning classification tools in the latest release of eCognition Developer 9.3.2.

17 September 2018 8,963 1 View

I am looking for a simple basic description of a system within systems theory used to describe an organisation?

I have studied systems theory in the more complex mathematical version and have a handout in Dutch describing an organisation as a system with the primary process and two kinds of feedback loops....

15 April 2018 6,746 2 View

Preparation of spheroids for microscopic analysis

I'm using HepG2 to form 3D HepG2 aggregates with alginate, to confocal microscopy analyzes. However, I have difficulty to maintaining the spheroids after fixation and all procedures of incubations...

24 July 2017 478 1 View

Does anyone work with esophageal cell line immortalized with hTERT ?

22 June 2016 3,575 3 View

Why does an H&E stained slide that showed the presence of calcium does not stain positive for von Kossa?

22 February 2015 9,571 4 View

ERK5 Ab to ChiP:

Does anyone use Anti ERK5 to Chromatin Immunoprecipitation assay (ChiP)? I want to start this protocol but dont know where I can find this Ab.

14 May 2013 2,365 1 View

Do I need to ask permission to use pictures for my questionnaire?

I would like to use some photo from a government website for my questionnaire. But, different photo is from the different owner. So, should I just get permission from that government body or still...

23 February 2021 1,631 3 View

Is recycling a challenge to the governments of developing countries?

Is recycling a challenge to the governments of developing countries? A strong will for more serious aggressively organized recycling initiatives, embedded with strict regulations supported through...

12 January 2021 510 13 View

I have started to design a Lithium ion battery in Comsol. I need to merge the LIB model with PDEs. any idea how to do that ?

the electrode structure has secondary pore networks , so I need to use different governing equations for the electrodes. I tried to use PDEs , but I have not get any results. Does anybody have any...

02 January 2021 3,225 1 View

How Inter-state water disputes affect unity and integration of India?

Water in India is in the State List and Union Government has the powers to constitute the Tribunals. Therefore, when a dispute emerges between two states it becomes difficult for the Union...

01 January 2021 9,309 3 View

How to use back-trajectory analysis (HYSPLIT) to assess air parcels at breathing/monitor height?

Hi there, I'm currently monitoring air pollution in Grenada, West Indies and as part of my project I have identified four scenarios in which I would like to use a back-trajectory analysis...

27 December 2020 730 4 View

Don't you have medical quacks claiming that they can cure COVID in your countries?

There's a quack who claims that he can cure patients with COVID using his 'magic portion'. Some media blindly support him, though there's no scientific evidence for his treatment. There's a...

17 December 2020 8,022 5 View

How weather based insurance is more effective than yield based?

In Nepal, crop insurance and refunding policy is is area-yield /revenue-based and cost of production based. Farmers are partially following crop insurance in comparison to livestock and other life...

07 December 2020 2,127 1 View

How can we construct a mathematical foundation of Leadership theory ( generally 4, 5, 8 etc. styles of Leadership ) using fuzzy system?

In the education discipline, several leadership theory has been discussed but no such mathematical foundations are available to estimate them. More, especially how can I differentiate( in...

17 November 2020 5,077 3 View

Is there a plausible explanation for the increased frequency of severe hurricanes in the southwest Atlantic Ocean other than climate change?

The Southeastern region of the United States of America, including Florida, Georgia, South Carolina, North Carolina, and the Caribbean archipelagos are being devastated by a higher number of...

16 November 2020 9,463 20 View

Where could I find open datasets on ransomware attacks that target councils and local governments, based in the UK and US?

For my assignment, I am to use open data that focuses on ransomware attacks (however other forms of cyber attacks are also useful) so that I can identify trends and write my report. My report is...

09 November 2020 2,093 5 View