What could be the reason of no transformation using a ligation mixture?

Recently, I have been working with some genes contained in pBlueScript II SK (+) and that contain XhoI and XbaI restriction sites. I would like to clone them in PET28a. For instance, the...

22 June 2020 3,474 5 View

What factors influence user acceptance of affective technologies?

Affective technologies are the interfaces concerning the emotional artificial intelligence branch known as affective computing (Picard, 1997). Applications such as facial emotion recognition...

08 May 2020 2,973 9 View

Are there zinc-CYS parameters in CHARMM files? of has anyone generated these parameters?

06 March 2020 5,939 2 View

How do I run a successful digestion for a large plasmid with GC Repeats, hairpins, and potentially other secondary structure?

I am doing some cloning and am having some issues with both sequencing my 22kb plasmid and running digestions with it. I sent my plasmid in for sequencing and got terrible results, but...

02 July 2019 1,506 3 View

How to perform KASP genotyping of zebrafish larvae at 72hpf?

Dear all, I need to genotype some zebrafish larvae at 3-4dpf. I currently tried to perform it as I do it for the adults, but it did not work. Basically: 1. Fin clip and add the fin to a 96 well...

20 January 2019 989 3 View

How to assess machine learning tools in eCognition Developer version 9.3.2?

How to assess machine learning classification tools in the latest release of eCognition Developer 9.3.2.

17 September 2018 8,963 1 View

No title

1.What is the maximum number of daily IP injections and 2. The maximum volume (per day & cumulated) tolerated in mice with a saline media (hydrophilic compound) and with DMSO (non-hydrophilic) ?

22 August 2018 3,772 1 View

I am looking for a simple basic description of a system within systems theory used to describe an organisation?

I have studied systems theory in the more complex mathematical version and have a handout in Dutch describing an organisation as a system with the primary process and two kinds of feedback loops....

15 April 2018 6,746 2 View

Why my protein isn't recognized by antibody after HPLC purification?

I got a solution that is highly recognized by ELISA, but after HPLC purification (C18 column), none of the elutions are recognized anymore. Is it possible that the Elution Buffer in some way made...

02 April 2018 9,916 10 View

Preparation of spheroids for microscopic analysis

I'm using HepG2 to form 3D HepG2 aggregates with alginate, to confocal microscopy analyzes. However, I have difficulty to maintaining the spheroids after fixation and all procedures of incubations...

24 July 2017 478 1 View

Is SSRN e-Journal Scopus indexed?

I have publication in SSRN. I want to know whether it is Scopus indexed? Does it show impact factor? What is its ISSN No.?

02 March 2021 4,565 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

How does one access the Cranet survey data on HRM practices?

Hello, The Cranet website does not provide any lead to the data obtained from their multinational survey on HRM practices. Any help on accessing the data would be most appreciated. Thank...

01 March 2021 9,536 3 View

Is there any research regarding how training and development is going to change in the era of Fourth Industrial Revolution?

As the organizations getting prepared for the fourth industrial revolution, to enhance the skills of their workforce what type of changes are necessary and going to occur in the training and...

01 March 2021 3,199 3 View

What is the most reliable source to get Impact factor of journals?

Source to get Impact factor of journals

01 March 2021 6,654 2 View

How long do vaccines protect us against COVID 19?

Or will we have to get vaccinated every year, as in the case of influenza?

27 February 2021 1,048 5 View

How to add missing citation?

Dear colleagues, I have found that many authors have a concern regarding their missing citation in RG because often citation in RG is lower than google scholars. I also missed some citation...

26 February 2021 928 6 View

How relevant is Mini slump cone?

for all the rheology researchers, my question is, how relevant is a mini slump cone (smaller than a standard 50 cent rheometer). currently based on testing, the thickness of slumped material is...

26 February 2021 3,668 1 View

Is IMPACT FACTOR justified in case of Taxonomy?

There is huge pressure of getting good impact factor on scholars now a days. On the other hand Taxonomy is a very dry subject and the concerned papers can only get citation in same group of organism.

26 February 2021 5,851 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View