there is no limitation for using the primers for a analytical purpose but to use it for cloning is something which is not advisable. so the answer depends on what is your application.
there is no limitation for using the primers for a analytical purpose but to use it for cloning is something which is not advisable. so the answer depends on what is your application.
RT PCR primers can be used for ordinary PCR. One just needs to be aware if they are intron-spanning. If so, how long is the intron(s)? Do you want only gel visualization of the amplicon, or intend to sequence the fragment? And that feeds into which sequencing method to use.