Is there any mouse Cell line producing CCL20/MIP3alpha?

07 August 2016 8,818 3 View

Can any one suggest protein linkers?

Can anyone suggest a eukaryotic linker for proteins with the property of auto cleavage or protease sensitive and size 3-6 amino acids.

03 April 2016 1,887 3 View

Why recombinant gene is not getting expressed?

I am trying to express fusion peptides of mycobacterium tuberculosis in BL21DE3 and Rosseta DE3 but not getting any expression. Vector I have used is pET23a and pET28b. Gene is fusion of 6...

02 March 2016 7,294 5 View

Is it possible to clone Promoter and RBS in to an expression vector?

I want to know that is there any possibility to clone complete promoter and ribosome binding site in a expression vector to increase the cloning site. I want to clone more than one gene to express...

10 November 2014 8,021 2 View

How can I carry out co-expression of mammalian gene into mycobacterium?

I want to express two mammalian gene into single plasmid of mycobacterium. In which way should these gene products should get secreted and separated? So which kind of strategy I should follow and...

07 August 2014 746 3 View

Restriction Ligation

I am trying to ligate my plasmid digested with BamH l and Hind lll and same to my gene of interest but I am unable to ligate them. I have used alkaline phosphatase digestion for plasmid but still...

11 December 2013 9,724 13 View

Which plasmid is used full to clone Mtb peptide and clone in M. smegmatis?

I want to produce some Mtb peptide in high amount and want to clone in M. smegmatis. Can anyone suggest the best plasmid for cloning and high expression?

09 October 2012 4,912 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

No title

02 March 2021 3,060 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View