Satya Deo Pandey

2 Questions 7 Answers 0 Followers

Questions related from Satya Deo Pandey

Why does aztreonam ineffective against gram-positive bacteria, Is the permeability a reason or poor affinity towards PBP3 of Gram +ive bacteria?

Can anyone explain, Why does aztreonam ineffective against gram-positive bacteria, Is the permeability a reason or poor affinity towards PBP3 of Gram +ive bacteria? please explain.

06 June 2017 2,543 4 View

A pET28a(+) bearing an insert is transformed into E. coli BL21DE3. But the protein is not expressing with conc. of IPTG and different temperature?

I have cloned a mycobacterial soluble fraction gene into pET28a(+) and transformed into E. coli BL21DE3. I tried 0.1, 0.2, 0.5 0.6, 0.8, 1.0 and 1.5 mM IPTG and 16, 20, 25 and 37C temp., but...

10 October 2015 8,075 5 View