Hello everyone
Is there any mouse cell line secreting CCL20/MIP3alpha?
Can we use human CCL20 in mouse for its bio-activity or not?
Hey
Check these links ,I hope helping you.
Neutrophils produce biologically active macrophage inflammatory ...
www.ncbi.nlm.nih.gov/.../11449350
National Center for Biotechnology Information
Macrophage inflammatory protein-3alpha (MIP-3alpha)/CCL20 and ... Animals; Cell Adhesion; Cell Line; Cells, Cultured; Chemokine CCL19; Chemokine CCL20 ... Macrophage Inflammatory Proteins/genetics; Mice; Mice, Inbred BALB C ...
CCL20 Displays Antimicrobial Activity Against Cryptosporidium ...
jid.oxfordjournals.org › .
Apr 2, 2015 - Intestinal Production of CCL20 by Neonatal Mice Is Reduced by Infection With C. .... CCL20 production by the mICcl2 cell line in response to ..... CCL20/MIP-3 alpha production by mouse uterine epithelial cells in culture.
CCL20 Displays Antimicrobial Activity Against Cryptosporidium ...
jid.oxfordjournals.org/content/.../infdis.jiv206.full.pdfThe Journal of Infectious Diseases
May 8, 2015 - The murine cell line mICcl2 was kindly provided by A. Vandewalle. [19] and was ... Intestinal Production of CCL20 by Neonatal Mice Is Reduced by Infection ..... CCL20/MIP-3alpha display antimicrobial activity. J Leukoc Biol ...
Mouse CCL20/MIP-3 alpha PicoKine ELISA Kit Clinisciences
www.clinisciences.com › Immunoassays › ELISA kits and sets › Human
Mouse MIP-3 alpha/CCL20 PicoKine ELISA Kit standard curve .... expressed in lymph nodes, appendix, PBL, fetal liver, fetal lung and several cell lines.
CCL20 - chemokine (C-C motif) ligand 20 - iHOP
www.ihop-net.org/UniPub/iHOP/bng/92052.html
Expression of the C-C chemokine MIP-3 alpha/ CCL20 in human epidermis ... CCL20/ MIP-3 alpha production by mouse uterine epithelial cells in culture. ..... expression of LARC was clearly detectable in all 12 glioma cell lines by RT-PCR.
Mouse CCL20/MIP-3 alpha Antibody MAB760-100: R&D Systems
https://www.rndsystems.com/.../mouse-ccl20-mip-3alpha-antibody-114...
R&D Systems
Neutralization. Measured by its ability to neutralize CCL20/MIP‑3 alpha -induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR6.
Good luck
CCL20 is a chemokine that works in tandem with MMP12. These two molecules are secreted simultaneously: the first ensures the attraction and the second allows migration.
Monocytes can produce large quantities of both molecules simultaneously. This requires that these monocytes are activated: a double activation is required: first the cells are cultured five days in GM + IL4, then these MODC are activated in the presence of IL10 + TNFa. Secretion of CCL20 is then between 1,000 and 10,000 times higher than that of PBL.
Most tumor cell lines can produce CCL20, but at a basal level.
If we compare the human and murine sequences of CCL20 are very close, however, the active region is rather variant. I'm not sure that human CCL20 may be perfectly functional on murine HEV, for example.
Thank you to both of you for your valuable information and suggestions.
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