I want to know that is there any possibility to clone complete promoter and ribosome binding site in a expression vector to increase the cloning site. I want to clone more than one gene to express them separately. I am using pJAK2.B vector.
Is there any paper regarding this kind of work?
Hi there,
What I do understand is that you want to modify a plasmid in order to be able to clone two different genes under the control of two different promoters. If the construction is properly done and if the final size is in the range where the plasmid is stable in vivo, it should work perfectly. As you mentioned, RBS has to be present at the right distance of initiation ATG and the presence of a transcription terminator downstream the stop codon will improve the whole system.
Hi Dominique
Yes I want to do the same as you have mentioned. Plasmid has the capability of insert upto 3 kb which should be helpful.
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