Wich cell lines express IDO/TDO enzymes and other Kynurenine pathway enzymes?

More Marzieh Rostaminejad's questions See All

How to dissolve Span 80 in mineral oil or liquid paraffin?

I want to work on microfluidic chip. Dispersed phase of alginate solution and continuous phase of Span 80 solution in combination with mineral oil or liquid paraffin. But I had a problem with the...

19 May 2024 1,790 7 View

Why we did not have clear bands with FluorChem protein simple imaging system?

we did not have clear bands with FluorChem protein simple imaging system .That is, the intensity of the bands are so high that the distance between the wells containing different samples are not...

26 January 2024 9,162 0 View

Should the new column from the subproblem always go into the base of the master problem in the next iteration?

In some iterations, when a new column is added to the master, it does not enter the base and a stop condition occurs, or a new column with a better objective function is found. is it correct? When...

20 November 2023 8,677 0 View

Can we consider incident rate ratio equal to hazard ratio in meta-analysis?

In a meta-analysis, there is just one study that reported the incident rate ratio, can I consider it as HR?

03 November 2023 1,843 4 View

Why my transduction efficiency doesn't exceed 10% with MOI=10 in Jurkat cells?

I am expressing two genes (CAR and a reporter) under EF-1a promoter in a lentiviral vector. I am using 2nd-generation lentiviral system (psPAX2 and VSV-G). My transfection efficiency in 293LTV...

24 October 2023 5,749 5 View

Whats the simplest method to measure OH radicals in a solution?

Hello everyone, I am currently involved in research focused on the electrochemical removal of synthetic wastewater as part of my project. I am seeking the simplest method to measure the OH...

04 October 2023 6,693 3 View

How to prevent the aggregation of positively charged nanoparticles in the aqueous environment?

I have made positively charged nanoparticles with Lecithin, Squalene, Tween 80, Span 85 and Dotap. But after using ultrasonic, the particles are aggregated and their size increases. How can...

10 September 2023 3,279 3 View

Can lipid nanoparticles larger than 200 nm pass through the 0.2 filter due to flexibility?

Hi, Can lipid nanoparticles larger than 200 nm pass through the 0.2 filter due to flexibility?

04 August 2023 2,726 2 View

Hi, what is the problem?

I made cationic nanocarrier by lecithin, Squalen, tween 80, span 85 and DOTAP via melting method in 80 C, but nanoparticles had negative charge instead of positive charge. Does anyone know where...

04 August 2023 5,741 1 View

What is the Maldi-tof technique?

What is the Maldi-tof technique? Is it necessary to perform Maldi-tof technique after protein purification?

16 June 2023 9,554 4 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,822 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,105 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 639 2 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,850 1 View

Can i quantify a nanoparticle encapsulated in cell culture medium by hplc?

Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...

03 August 2024 2,730 1 View