Does the amount of ammonium measured by the electrode depend on temperature?

19 November 2020 6,315 12 View

I am seeking for a Questionnaire which is in this book: Consumption Values and Market Choices: Theory and Applications, does anyone have access to it?

For my new research, I need a questionnaire regarding consumption value theory (specially conditional value questions), Sheth 1991 has a standard questionnaire in his book which is named...

02 May 2020 731 2 View

What do you think about resilient supply chain?

According to the spreed of COVID-19 ,many supply chains all around the world, have been affected by negative impacts of this virus, especially in countries with the highest prevalence. In this...

11 March 2020 7,035 9 View

Is there any reference value that can be compared to the obtained health risk index (HRI)?

In order to evaluate the risk from tomato ingestion in the presence of organic wastes, health risk index (HRI) of heavy metals in each treatment were calculated . Is there any reference value...

14 July 2019 8,279 1 View

What is the probable reason for mismatch between results of fluorescent microscopy and flow cytometry?

I used a GFP vector to evaluate the transfection efficiency. the negative control, is a GFP-free vector. results of Fluorescent microscopy show transfection at rates higher than 90%. But the...

20 September 2018 1,346 6 View

Correlation coefficent of two catagorical variables

Hello, Is there anyway to have the correlation coefficient of two categorical variables ,both ordinal and nominal (For example level of education and ethnicity)? I have checked the independency by...

30 July 2017 3,229 3 View

Phosphoserine simulation using Gromacs

Hi Dear all I want to simulate a protein containing phosphoserine using MD, so I place the force field into the working directory and modified all parameters such as SEP (phosphoserine) was added...

15 June 2017 960 8 View

Can anyone help me about PCR of DNA extrected from Mia-paca-2 cell line?

Dear all, I should extract DNA from some cancer cell lines and perform PCR for its. I did it by Qiagen kit. From among all, PCR of DNA from Mia-paca-2 cell line (cell line of Pancreatic cancer)...

23 September 2015 6,523 3 View

I want to test the effect of lacking a gene on male infertility. How many male I should mate? and how many female I have to mate with the KO male?

06 August 2015 3,293 1 View

Which kit is more accurate for determination of protein concentration in SDS- Page sample buffer?

Which kit is more accurate for determination of protein concentration in SDS- Page sample buffer?1- Protein assay kit (Bio-RAD)2- 660 nm protein assay (Thermo scientific)3- 2-D quant kit (GE...

20 August 2014 9,614 10 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View