Hi,
I know that we can use glycols instead of DMSO which can be less toxic but what are the advantages of DMSO over other cryopreservation agents that make us preferably use DMSO?
DMSO helps to prevent water crystals from forming, which can burst cell membranes. This is what I remember.
DMSO is well tolerated by several types of cells even at relatively high concentrations (around 0.1%).
Hi Reyhane, Your question has already been discussed at various places before. I am giving reply followed by links for your detailed understanding.
DMSO is most often used at a final concentration of 5 to15% (v/v). Some cell lines are adversely affected by prolonged contact with DMSO. This can be reduced or eliminated by adding the DMSO to the cell suspension at 4°C and removing it immediately upon thawing. If this does not help, lower the concentration or try glycerol.
Glycerol is generally used at a final concentration of between 5 and 20% (v/v). Although less toxic to cells than DMSO, glycerol can cause osmotic problems, especially after thawing. Always add it at room temperature or above, and remove slowly by dilution.
-https://www.researchgate.net/post/Why_is_DMSO_rather_than_Glycerol_used_while_preparing_cells_for_cryo_and_why_is_glycerol_used_preferentially_while_storing_microbial_culture
-https://www.researchgate.net/post/Can_we_use_glycerol_to_freeze_cells_instead_of_DMSO
-https://www.intechopen.com/books/cryopreservation-biotechnology-in-biomedical-and-biological-sciences/cryoprotectants-and-their-usage-in-cryopreservation-process
-Article Comparison of DMSO and glycerol as cryoprotectants for ascit...
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