Hi,
I have a simple question: When we freeze cells why we use 90% FBS and 10% DMSO?
I know that DMSO is used for cell cryopreservation but I don't know why we use FBS instead of culture media?
So it's not technically NOT true that you can't use cell media with FBS, you can. But cells revive better if it's in 90% FBS. As to why? I just figure that there are higher concentration of proteins in 90% FBS than media/FBS that helps with the cells going through a freeze thaw cycle.
In many cases, cells freezed in 10% DMSO and 90% FBS have better survival after thawing them when compared to cells cryopreserved in less FBS concentration (10%). However, since FBS is quite expensive, using 10% FBS for cryopreservation (80% media and 10% DMSO) is still a good option. I´ve seen around 90-95% viability using this concentrations when cryopreserving breast cancer cell lines.
Mauricio de la Rosa Shen-An Hwang Thank you, I appreciate your help. I got my answer!
- Badges
- Science topic