hello all,

i am confused with result i got in CN-native PAGE.

basically i am working on two proteins which are involved in a pathway.

first i did activity assay in which protein A(1st protein) binds to substrate and forms a product which is utilized by protein B(2nd protein) and form final product that i detected with ATPase assay.

then i checked interaction of these protein with each other by SPR and i got binding affinity 5uM.

That means protein A is interacting with B and forming complex.

but i did not get any complex band in native page

can you help me out this puzzling condition?