Ketul Saharan

3 Questions 10 Answers 0 Followers

Questions related from Ketul Saharan

Why we are unable to get complex of two protein in CN-native PAGE though we got 5uM binding affinity through Surface Plasmon Resonance (SPR)?

hello all, i am confused with result i got in CN-native PAGE. basically i am working on two proteins which are involved in a pathway. first i did activity assay in which protein A(1st protein)...

07 July 2018 5,684 0 View

I am getting problem in protein expression.will codon optimization work for my case?

hello all, i am trying to clone and express M. tuberculosis protein in E. coli arctic express cell line. i got expression in soluble fraction(40%) during small scale expression test but...

07 July 2018 8,078 9 View

Why more bands are coming in pellet(dissolved in 8M urea) fraction rather than soluble fraction after SDS PAGE?

I was doing expression test for a protein. Of gene which I cloned in pETM41 vector and expressing in E. Coli system. When I grown the culture at 20 degree Celsius induced with .5 ,0.25, .125 mM....

06 June 2018 9,062 10 View