thanks in advance
I have qRT-PCR data of 4 transgenic line and 1 non-transgenic line. Non-trangenic line has GOI Ct value zero and HKG has Ct value because non-transgenic has no transgene/GOI, that's why no Ct...
05 June 2018 7,773 4 View
I have a construct having Restriction site SmaI+KpnI on both sides of gene. I want to lift the gene with SmaI and KpnI. SmaI linearized it, after it i purify with PCR kit and digest with KpnI....
08 September 2017 9,202 5 View
i am amplifying a gene which is GC rich (5' highly GC rich ~76% and 3' ~66%). I have non-specific amplification but no desired band till now. I have done/used 1. gradient PCR 2. DMSO etc. but...
01 February 2017 5,941 6 View
there are many constitutive promoter but expression level in all tissue is not equal. any promoter which also give high expression level in seed and pollen? if any relevant paper then please...
10 November 2016 4,673 3 View
If i want to make a transgenic plant to express human protein or other, having secretory path just like Bombyxin or insulin then which signal peptide (plant origin ) should be attached So, that...
10 November 2016 3,162 1 View
A gene (high level GC content 70 % as compared to other host gene )which is native and when express it under constitutive promoter in plant, not give high expression. what may be the cause?
09 October 2016 1,218 1 View
glycosylated protein. codon optimzation in such a way, that properly glycosylated and properly folded. what things should i ocnsider during codon optimzation
09 October 2016 693 3 View
Hello, I suspect that a molecule I am using is causing bacteria to experience osmotic shock. What chemical properties should I look for to compare this capability with those of other substances?...
06 August 2024 977 1 View
We are working on biopolymeric hydrogels. Our system is highly viscous and sticky, and the gel formed are high in strength. We are unable to use pH electrode and pH strip. Please suggest an easy...
30 July 2024 942 2 View
Hello everyone! How can i prepare EDTA buffer solution (pH 8.2) to determine GSH levels? Thank you in advance.
29 July 2024 4,374 1 View
I want to preserve the sample leaves for the LM study of the stomata and conidia analysis. Whether 2.5% glutaraldehyde in 0.05 M phosphate buffer with pH 7 can be applied for preservation.
29 July 2024 8,560 0 View
The column is used in Ion Exchange Chromatography of Monoclonal antibodies and the method of analysis is based on pH gradint.
13 July 2024 7,462 1 View
I performed CV of Cu-MOF in a buffer solution of pH 8, and we did not find any peak corresponding to Cu2+ to Cu+. What is the reason behind this? Please give your valuable suggestions.
08 July 2024 2,388 4 View
I was wondering if it is possible to form a permanent open "ssDNA bubble" similar to a transcription bubble (>13 nucleotides) within E. coli. These criteria are important: 1. Open ssDNA...
07 July 2024 8,275 5 View
Can this pH of 8 be due to the presence of other substances? Or do I have an error somewhere in the analysis? Thank you!
26 June 2024 9,093 4 View
The percentage molar concentration of lactic acid and CTAB
20 June 2024 3,225 0 View
Hello, We are designing a protocol to determine the effect of certain compounds in the health of rats, but our problems have certain instability issues when subjected to certain pHs. We were...
19 June 2024 5,950 1 View
