How to analyze qRT-PCR data by using delta delta Ct method when one sample has Ct value of GOI Zero?

I have qRT-PCR data of 4 transgenic line and 1 non-transgenic line. Non-trangenic line has GOI Ct value zero and HKG has Ct value because non-transgenic has no transgene/GOI, that's why no Ct...

05 June 2018 7,664 4 View

Why SmaI is not digesting the plasmid completely?

I have a construct having Restriction site SmaI+KpnI on both sides of gene. I want to lift the gene with SmaI and KpnI. SmaI linearized it, after it i purify with PCR kit and digest with KpnI....

08 September 2017 8,995 5 View

How to amplify a GC-rich gene from genome?

01 February 2017 5,841 6 View

Any constitutive promoter which give high expressio level also in endosperm?

10 November 2016 4,541 3 View

which signal peptide should be used to target the (human or other) protein extracellular in transgenic plant? (secretory path like Bombyxin/insulin)

10 November 2016 3,072 1 View

What is the effect of high level GC content in a gene, on its expression level?

A gene (high level GC content 70 % as compared to other host gene )which is native and when express it under constitutive promoter in plant, not give high expression. what may be the cause?

09 October 2016 1,077 1 View

What points shoud be considered during codon optimization of glycosylated protein?

glycosylated protein. codon optimzation in such a way, that properly glycosylated and properly folded. what things should i ocnsider during codon optimzation

09 October 2016 606 3 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

Which database would give me this list of chemical components?

Dear colleagues: I would like to know if anyone knows of a database that gives me the parameters pH, K, K, Mg, P, C, N, Zn, Cu, Ni and Cd of common foods. I find a lot of information of proteins,...

26 February 2021 5,525 3 View

Why is NH4OH added in heavy metals limit test?

The limit test I'm referring to is USP method II. In this procedure you need to dropwise add 6N of NH4OH to the sample until it the pH is alkali. After that you need to dilute and add HOAc until...

23 February 2021 2,049 3 View

Is there an ideal incubation time of RGO-modified SPCE before subsequent use, i.e., electrochemical characterization or further modification?

I notice that upon washing and air-drying of my RGO/SPCE surface and characterizing again via DPV, the current response towards ferricyanide probe significantly decreases. My worry is that the...

22 February 2021 5,324 3 View

No title

22 February 2021 9,932 11 View

Can anyone help or comment on solubilising chlorobutanol in aqueous injections in minimum time possible?

Please suggest solubilisation of chlorobutanol in injections. Tried pH and high temperature. But the issue is of assay drop. If anyone can suggest any solution. Its a commonly used excipient for...

21 February 2021 7,397 1 View

What reaction will occur when adding hexamine into ammonium nitrate in an acidic medium?

Hello, I am investigating properties of water in oil emulsion explosives. And adding hexamine (hexamethylenetetramine) into one of the phases that are used to make the emulsion should increase the...

21 February 2021 8,680 1 View

What properties of water shall be analyzed and evaluated to test the effectiveness of activated carbon?

I would like to ask what other properties of water (pH and such) shall be analyzed and evaluated to test the effectiveness of activated carbon from filtering the contaminants present in the water?

20 February 2021 9,422 8 View

Phenol-pH graphic?

We want to use phenol pH graphic to measure the pH of mediums before and after inhibitor application to cells. We have phenol absorbence values at 460, 560 and 720 nm wavelength. When we measure...

20 February 2021 7,700 3 View

How to measure the pH of Peat Soil?

Peat possess organic content above 75% and is highly acidic in nature (i.e. pH < 4). Before commencing stabilization, it is mandatory to know about the pH level of peat. Therefore, your...

18 February 2021 9,424 7 View