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Questions related from Naveed Anjum
I have qRT-PCR data of 4 transgenic line and 1 non-transgenic line. Non-trangenic line has GOI Ct value zero and HKG has Ct value because non-transgenic has no transgene/GOI, that's why no Ct...
06 June 2018 7,738 4 View
I have a construct having Restriction site SmaI+KpnI on both sides of gene. I want to lift the gene with SmaI and KpnI. SmaI linearized it, after it i purify with PCR kit and digest with KpnI....
09 September 2017 9,116 5 View
i am amplifying a gene which is GC rich (5' highly GC rich ~76% and 3' ~66%). I have non-specific amplification but no desired band till now. I have done/used 1. gradient PCR 2. DMSO etc. but...
02 February 2017 5,907 6 View
If i want to make a transgenic plant to express human protein or other, having secretory path just like Bombyxin or insulin then which signal peptide (plant origin ) should be attached So, that...
11 November 2016 3,135 1 View
there are many constitutive promoter but expression level in all tissue is not equal. any promoter which also give high expression level in seed and pollen? if any relevant paper then please...
11 November 2016 4,636 3 View
glycosylated protein. codon optimzation in such a way, that properly glycosylated and properly folded. what things should i ocnsider during codon optimzation
10 October 2016 668 3 View
A gene (high level GC content 70 % as compared to other host gene )which is native and when express it under constitutive promoter in plant, not give high expression. what may be the cause?
10 October 2016 1,177 1 View
thanks in advance
05 May 2015 4,627 0 View
