The percentage molar concentration of lactic acid and CTAB
Data file -> Which contains some tabular data. It can be a json or any file type but contains structural data. Config file -> Which contains the configurations of the project in any file...
05 December 2019 3,971 2 View
Dear Dr. Lin, I am working on the effect of wheat gluten hydrolysate as a biostimulant on sugarbeet growth. But, I need to know what physiological and biochemical parameters do you measure. I...
28 April 2019 7,110 5 View
I am trying to carry out Linear polyethyleneimine (PEI) mediated transfection on HEK293T cells. I also tried the same with NIH3T3 cells. I am using a 3:1 PEI to DNA ratio. However, I find my...
01 January 1970 9,018 2 View
Hello, Can anyone provide me with the absorption coefficient of methane gas at 7.7 um? Any reference?
06 August 2024 980 5 View
How to calculate the molar proportions of the oxides from the XRF analyses?
02 August 2024 2,565 2 View
NA
11 July 2024 8,969 1 View
I want to predict the DAR ratio based on the number of cys available for conjugation to Cys residue. I use Ellman's assay to predict the concentration of Cys after TCEP reduction. I gain a...
02 July 2024 612 6 View
I'm currently working on preparing a 10 mL liposome solution containing HSPC, mPEG2000-DSPE, and cholesterol at a molar ratio of 55/40/5. can anybody advise on the best way to calculate the weight...
26 June 2024 1,135 0 View
I am doing a DNA extraction from potato leaves infected with Phytophthora infestans for quantification via qPCR. The protocol includes two buffers: first, the tissue is disgragated with an...
19 June 2024 8,609 1 View
Good evening, I´m establishing a protocol for detecting ROS using DHE staining in Flow Cytometry using TK6 cells. They look visibly pink after staining them with 10 micro Molar DHE. Is that...
19 June 2024 7,339 0 View
Greetings, esteemed academics, I would want to question about the process of extracting DNA from the thoracic region of honey bees using CTAB. I have conducted this experiment on multiple...
14 June 2024 5,568 2 View
I am using CTAB method (Naser M. Salameh et al., 2014) for fungal DNA isolation and purification. During reagents preparation I am not sure weather I should autoclave 2X CTAB and Wash buffer. So...
09 June 2024 8,470 2 View
Hi, I have been trying to extract Plant genomic DNA via CTAB method and I am getting a 260/280 ratio ranging between 1.3 to 1.6. Any idea how to modify the protocol for fabaceae family plant...
03 June 2024 7,722 6 View