I have prepared DNA libraries; the fragment size is about 300 to 700 bp. In the sequencing results, I see the Mean insert size is 42bp.?

The sequencing platform used was Novaseq PE150. DNA Libraries prepared by KAPA.

QC was performed using tapstation. (Attached)

This has resulted in low coverage (0.2) with 250M reads. Alignment % is low at 34% while duplication is 12%.

The genome size is 3.5GB.

#Sequencing #DNA #WholeGenomeSequencing

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